Spatial control of DNA reaction networks by DNA sequence.

Spatial control of DNA reaction networks by DNA sequence.
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DOI:
10.3390/molecules171113390
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发表时间:
2012-11-09
期刊:
Molecules (Basel, Switzerland)
影响因子:
--
通讯作者:
Ellington AD
Ellington AD
中科院分区:
其他
文献类型:
--
作者:
Allen PB;Chen X;Ellington AD

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我们已经开发了一套DNA电路,在凝胶电泳过程中执行,以在凝胶中产生固定的荧光特征。正交电路的并行执行导致在凝胶中的不同位置处同时产生不同的荧光线。通过改变反应物的迁移率,可以合理地控制谱线的位置。在纳米尺度上编程以便在宏观尺度上产生图案的能力是朝着用于产生限定的时空图案的可编程合成化学系统迈出的一步。
We have developed a set of DNA circuits that execute during gel electrophoresis to yield immobile, fluorescent features in the gel. The parallel execution of orthogonal circuits led to the simultaneous production of different fluorescent lines at different positions in the gel. The positions of the lines could be rationally manipulated by changing the mobilities of the reactants. The ability to program at the nanoscale so as to produce patterns at the macroscale is a step towards programmable, synthetic chemical systems for generating defined spatiotemporal patterns.
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