Prognostic significance of GAD1 overexpression in patients with resected lung adenocarcinoma
Prognostic significance of GAD1 overexpression in patients with resected lung adenocarcinoma
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GAD1过表达在肺腺癌切除患者中的预后意义
DOI：
10.1002/cam4.2345
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发表时间：
2019
期刊：
影响因子：
4
通讯作者：
Tangoku A
中科院分区：
文献类型：
--
作者：
Tsuboi M;Kondo K;Masuda K;Tange S;Kajiura K;Kohmoto T;Takizawa H;Imoto I;Tangoku A
背景和目的在以前的全基因组筛选中，我们发现肺腺癌（LADC）中谷氨酸脱羧酶1（GAD 1）周围的CpG岛高度甲基化。本研究旨在探讨GAD 1的甲基化和表达状态及其在LADC患者中的预后价值。GAD 1过表达的预后价值进行了研究，使用公开可用的数据集的mRNA水平和162例LADCs-immunohistochemistry.ResultsThe甲基化和GAD 1的mRNA表达水平，每一个有正相关性，在LADC肿瘤显着高于配对的非肿瘤组织。在公开数据集中，GAD 1 mRNA表达较高的LADC患者的总生存期预后显着较差。GAD 1的高免疫反应性与病理分期、胸膜浸润、淋巴管浸润以及癌症特异性和无病生存期的不良预后显著相关。结论GAD 1 mRNA和蛋白表达水平是影响LADC患者预后的重要因素，可作为判断LADC患者术后预后的重要指标。
Background and ObjectivesIn a previous genome‐wide screening, we identified hypermethylated CpG islands aroundglutamate decarboxylase 1(GAD1) in lung adenocarcinoma (LADC). In this study, we aimed to investigate the methylation and expression status of GAD1 and its prognostic value in patients with LADC.MethodsGAD1methylation and mRNA expression status were analyzed using 33 tumorous and paired non‐tumorous LADC samples and publicly available datasets. The prognostic value of GAD1 overexpression was investigated using publicly available datasets of mRNA levels and 162 cases of LADC by immunohistochemistry.ResultsThe methylation and mRNA expression levels of GAD1, each having a positive correlation, were significantly higher in LADC tumors than in paired non‐tumorous tissues. LADC patients with higher GAD1 mRNA expression showed significantly poorer prognosis for overall survival in publicly available datasets. Higher immunoreactivity of GAD1 was significantly associated with the pathological stage, pleural invasion, lymph vessel invasion, and poorer prognosis for cancer‐specific and disease‐free survival. Multivariate analysis revealed that GAD1 protein overexpression is an independent prognosticator for disease‐free survival.ConclusionsGAD1 mRNA and protein expression levels were significant prognostic factors in LADC, suggesting that they might be useful biomarkers to stratify patients with worse clinical outcomes after resection.
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在早期肺腺癌中，启动子甲基化导致候选肿瘤抑制因子 TSLAC1 频繁沉默。
DOI：
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发表时间：
2016
期刊：
影响因子：
--
作者：
Kohmoto T;Kajiura K;Naruto T,Masuda K、Kondo K;Tangoku A;Imoto I.
通讯作者：
Imoto I.
影响因子：
11.2
作者：
Schnepp PM;Lee DD;Guldner IH;O'Tighearnaigh TK;Howe EN;Palakurthi B;Eckert KE;Toni TA;Ashfeld BL;Zhang S
通讯作者：
Zhang S
影响因子：
2.8
作者：
Mylona, E;Kapranou, A;Nakopoulou, L
通讯作者：
Nakopoulou, L
影响因子：
10.3
作者：
Shigematsu, H;Lin, L;Gazdar, AF
通讯作者：
Gazdar, AF
DOI：
10.1152/physiol.00002.2009
发表时间：
2009-06
期刊：
Physiology (Bethesda, Md.)
影响因子：
--
作者：
Young SZ;Bordey A
通讯作者：
Bordey A