Engineering cellular communication between light-activated synthetic cells and bacteria.
Engineering cellular communication between light-activated synthetic cells and bacteria.
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DOI：
10.1038/s41589-023-01374-7
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发表时间：
2023-09
影响因子：
14.8
通讯作者：
Booth, Michael J.
中科院分区：
文献类型：
--
作者：
Smith, Jefferson M.;Hartmann, Denis;Booth, Michael J.
基因表达区室由简单的模块化部件组装而成，是用于创建具有类似生命功能的最小合成细胞的通用平台。通过将基因调控基序整合到其封装的DNA模板中，可以根据特定的刺激来控制原位基因表达，从而控制合成细胞功能。在这项工作中，通过在光激活的DNA模板上编码感兴趣的基因，利用光控制合成细胞内的无细胞蛋白质合成。光激活的DNA在T7启动子区域内含有可光裂解的阻断剂，该阻断剂紧密抑制转录，直到阻断基团被紫外光去除。通过这种方式，合成细胞以时空控制的方式被远程激活。通过将这种策略应用于酰基高丝氨酸内酯合酶BjaI的表达，合成细胞和细菌之间基于群体感应的通信用光来控制。这项工作提供了一个框架，远程控制的生产和交付的小分子从非生命物质的生命物质，在生物学和医学的应用。合成细胞，即模块化基因表达隔室，在生物学和医学中已显示出广阔的应用前景;然而，它们的控制和通讯需要更多样化的工具。现在，光笼启动子和酰基高丝氨酸内酯的无细胞合成已被用于证明合成细胞和活细胞之间的光激活通信。
Gene-expressing compartments assembled from simple, modular parts, are a versatile platform for creating minimal synthetic cells with life-like functions. By incorporating gene regulatory motifs into their encapsulated DNA templates, in situ gene expression and, thereby, synthetic cell function can be controlled according to specific stimuli. In this work, cell-free protein synthesis within synthetic cells was controlled using light by encoding genes of interest on light-activated DNA templates. Light-activated DNA contained a photocleavable blockade within the T7 promoter region that tightly repressed transcription until the blocking groups were removed with ultraviolet light. In this way, synthetic cells were activated remotely, in a spatiotemporally controlled manner. By applying this strategy to the expression of an acyl homoserine lactone synthase, BjaI, quorum-sensing-based communication between synthetic cells and bacteria was controlled with light. This work provides a framework for the remote-controlled production and delivery of small molecules from nonliving matter to living matter, with applications in biology and medicine. Synthetic cells, modular gene-expressing compartments, have shown promising applications in biology and medicine; however, more diverse tools are required for their control and communication. Now, photocaged promoters and cell-free synthesis of an acyl homoserine lactone have been used to demonstrate light-activated communication between synthetic cells and living cells.
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影响因子：
14.9
作者：
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通讯作者：
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