Geometry and force control of cell function.
Geometry and force control of cell function.
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DOI：
10.1002/jcb.22355
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发表时间：
2009-12-01
影响因子：
4
通讯作者：
Vunjak-Novakovic, Gordana
中科院分区：
文献类型：
--
作者：
Freytes, Donald O.;Wan, Leo Q.;Vunjak-Novakovic, Gordana
组织工程在创造功能性人体组织和为干细胞科学家提供高度生物保真度的培养系统方面正变得越来越雄心勃勃。新的工程设计正受到生物学原理的指导，试图更忠实地再现天然细胞环境的复杂性。三维（3D）支架被设计成模拟天然样细胞环境，从而引发天然样细胞反应。此外，传统的重点是分子调控因子正在转向分子和物理因素的联合应用。最后，方法正在成为可用的分子和物理因素的可控的空间和时间梯度的形式协调的介绍。总之，这些最新的发展使得能够在动态培养环境中询问细胞行为，该动态培养环境旨在模拟天然组织发育、疾病或再生的某些方面。我们在这里讨论这些先进的细胞培养环境，重点是从发展（仿生范式）和细胞功能的几何力控制（生物物理调节范式）的设计原则的推导。
Tissue engineering is becoming increasingly ambitious in its efforts to create functional human tissues, and to provide stem cell scientists with culture systems of high biological fidelity. Novel engineering designs are being guided by biological principles, in an attempt to recapitulate more faithfully the complexities of native cellular milieu. Three-dimensional (3D) scaffolds are being designed to mimic native-like cell environments and thereby elicit native-like cell responses. Also, the traditional focus on molecular regulatory factors is shifting towards the combined application of molecular and physical factors. Finally, methods are becoming available for the coordinated presentation of molecular and physical factors in the form of controllable spatial and temporal gradients. Taken together, these recent developments enable the interrogation of cellular behavior within dynamic culture settings designed to mimic some aspects of native tissue development, disease, or regeneration. We discuss here these advanced cell culture environments, with emphasis on the derivation of design principles from the development (the biomimetic paradigm) and the geometry-force control of cell function (the biophysical regulation paradigm).
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DOI：
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发表时间：
2000-12-01
影响因子：
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期刊：
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