Conformational properties of the main intrinsic polypeptide (MIP26) isolated from lens plasma membranes.
Conformational properties of the main intrinsic polypeptide (MIP26) isolated from lens plasma membranes.
复制标题
从晶状体质膜分离的主要内在多肽 (MIP26) 的构象特性。
作者：
Horwitz,J;Bok,D
修订稿于 1987 年 7 月 28 日收到 摘要：通过使用近紫外和远紫外圆二色性研究了从晶状体质膜中分离出的主要内在多肽 (MIP26) 的构象特性。用辛基3-D-吡喃葡萄糖苷溶解的MIP26的远紫外光谱表明α-螺旋含量为~50%，3-结构含量为~20%。 MIP26 的无洗涤剂膜悬浮液产生了典型的扭曲远紫外光谱，这是由微分光散射和吸收平坦化引起的。然而，通过超声处理减小膜碎片的尺寸产生了无畸变的远紫外光谱，并且具有与用辛基3-D-吡喃葡萄糖苷溶解的MIP26获得的相似的旋转强度分布。这意味着悬浮液和糖去污剂中的蛋白质具有相似的二级结构特性。用金黄色葡萄球菌蛋白酶切割 MIP26，导致去除 5 千道尔顿的肽，并模拟晶状体中发生的年龄依赖性翻译后变化，根据近紫外圆二色光谱判断，并没有显着影响核心蛋白的构象。脊椎动物的晶状体是一种具有高度有序结构的无血管组织（Maisel 等，1981；Benedetti 等，1981）。晶状体的大部分由多个 f 组成。这项工作部分得到了 USPHS 资助 EY 00331、EY00444 和 EY 03897 的支持。DB 是 Dolly Green 眼科教授。细长的、不分裂的细胞，称为晶状体纤维。这些晶状体纤维通过位于其质膜上的许多细胞间连接点彼此耦合（Rae，1979；Goodenough 等，1980；Benedetti 等，1981）。晶状体纤维质膜中最丰富的蛋白质是称为 MIP26 或 MP26 的内在膜蛋白（Bro-ekhuyse 等，1976；Benedetti 等，1981）。这种蛋白质，
Revised Manuscript Received July 28, 1987 abstract: The conformational properties of the main intrinsic polypeptide (MIP26) isolated from lens plasma membranes were studied by using near- and far-ultraviolet circular dichroism. The far-ultraviolet spectrum of MIP26 solubilized with octyl 3-D-glucopyranoside indicates an a-helical content of~ 50% and a 3-structure content of~ 20%. A detergent-free membrane suspension of MIP26 produced a typically distorted far-ultraviolet spectrum which was caused by differentiallight scattering and absorption flattening. However, decreasing the size of the membrane fragments by sonication produced a far-ultraviolet spectrum free of distortion, and with a rotatory strength profile similar to that obtained for MIP26 solubilized with octyl 3-D-glucopyranoside. This implies similar secondary structure properties for the protein in both the suspension and the sugar detergent. The cleavage of MIP26 with Staphylococcus aureus protease, which results in removal of a 5-kilodalton peptideand which mimics theage-dependent posttranslational changes that takeplace in the lens, did not significantly affect the conformation of the core protein as judged by the near-ultravioletcircular dichroism spectra. e vertebrate eye lens is an avascular tissue with a highly ordered architecture (Maisel et al., 1981; Benedetti et al., 1981). The bulk of the lens is composed of a multitude of f This work was supported in part by USPHS Grants EY 00331, EY00444, and EY 03897. DB is the Dolly Green Professor of Ophthalmology. elongated, nondividing cells which are called lens fibers. These lens fibers are coupled to one another by many intercellular junctions situated along their plasma membranes (Rae, 1979; Goodenough et al., 1980; Benedetti et al., 1981). The most abundant protein in the lens fiber plasma membrane is an intrinsic membrane protein known as MIP26 or MP26 (Bro-ekhuyse et al., 1976; Benedetti et al., 1981). This protein,
登录
查看更多内容
小牛晶状体纤维内膜蛋白 MP26 及其天然成熟产物 MP22 肽片段的序列分析
影响因子：
3.5
作者：
L. D. Ngoc;P. Paroutaud;I. Dunia;E. Benedetti;J. Hoebeke
通讯作者：
J. Hoebeke
从圆二色光谱估计蛋白质二级结构的计算机辅助模型：动物乳酸脱氢酶的比较。
影响因子：
2.9
作者：
Jeffrey B. Siegel;Wayne E. Steinmetz;George L. Long
通讯作者：
George L. Long
校正吸收颗粒悬浮液中的吸收平坦和散射的实验方法：红细胞中原位血红蛋白的圆二色性和吸收光谱。
影响因子：
2.9
作者：
A. S. Schneider;D. Harmatz
通讯作者：
D. Harmatz
影响因子：
3.4
作者：
BROEKHUYSE, RM;KUHLMANN, ED;STOLS, ALH
通讯作者：
STOLS, ALH
DOI：
10.1083/jcb.86.2.576
发表时间：
1980-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Goodenough DA;Dick JS 2nd;Lyons JE
通讯作者：
Lyons JE