The role of SPARC protein expression in the progress of gastric cancer.
The role of SPARC protein expression in the progress of gastric cancer.
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DOI：
10.1007/s12253-012-9497-9
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发表时间：
2012-07
影响因子：
2.8
通讯作者：
Sun, Wenguang
中科院分区：
文献类型：
--
作者：
Wang, Lifeng;Yang, Miling;Shan, Lihui;Qi, Lei;Chai, Cuicui;Zhou, Qiufeng;Yao, Ke;Wu, Hongmei;Sun, Wenguang
本研究旨在探讨富含半胱氨酸的酸性分泌蛋白（secreted protein，acidic and rich in cysteine）在胃癌组织中的表达及其与肿瘤血管生成和癌细胞增殖的关系。采用免疫组化法检测80例胃癌组织和30例正常胃组织中VEGF、CD 34和Ki-67的表达。CD 34染色作为微血管密度（MVD）的指标。Ki-67标记指数（LI）表示癌细胞增殖。统计分析其与临床特征、肿瘤血管生成及肿瘤细胞增殖的关系。胃癌组织和正常胃组织中，TGF β 1主要表达于癌细胞周围的间质细胞，差异有统计学意义（P < 0.05）。VEGF和VEGF的表达与胃癌的分化程度、临床分期、Lauren分级及淋巴结转移有关（P < 0.05）。胃癌组织中VEGF和MVD的表达与VEGF的表达呈显著负相关。Ki-67-LI与PCNA表达呈负相关。结果提示，胃癌组织中VEGF和VEGF的表达与肿瘤血管生成密切相关，VEGF抑制肿瘤血管生成，而VEGF促进肿瘤血管生成。对胃癌细胞增殖也有抑制作用。
We aimed to investigate the expression of SPARC (secreted protein, acidic and rich in cysteine) in gastric cancer and its relationship with tumor angiogenesis and cancer cells proliferation. Protein expression of SPARC, VEGF, CD34 and Ki-67 in 80 cases of gastric cancer and 30 cases of normal gastric tissue was evaluated by immunohistochemistry. CD34 staining was used as an indicator of microvessel density (MVD). Ki-67 labeling Index (LI) indicated cancer cells proliferation. Statistical analysis was used to investigate its relationship with clinical characteristics, tumor angiogenesis and cancer cells proliferation. SPARC expression was mainly in the stromal cells surrounding the gastric cancer cells, and was statistically significant differences between gastric cancer and normal gastric tissue (P < 0.05). Both the expression of SPARC and VEGF were related to differentiation degree, clinical stage, Lauren classification and lymph node metastasis (P < 0.05). Expression of SPARC was significantly negatively correlated with the expression of VEGF and MVD in gastric cancer tissues. Expression of SPARC was also negatively correlated with Ki-67-LI. Our findings suggest that both the expression of SPARC and VEGF are closed to tumor angiogenesis in gastric cancer, SPARC inhibited tumor angiogenesis but VEGF promoted tumor angiogenesis. SPARC also inhibited cells proliferation of gastric cancer.
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影响因子：
6.4
作者：
Yunker, Christopher K.;Golembieski, William;Rempel, Sandra A.
通讯作者：
Rempel, Sandra A.
影响因子：
15.9
作者：
Bradshaw, AD;Sage, EH
通讯作者：
Sage, EH
DOI：
10.1073/pnas.89.4.1281
发表时间：
1992-02-15
影响因子：
11.1
作者：
RAINES, EW;LANE, TF;SAGE, EH
通讯作者：
SAGE, EH
影响因子：
10.3
作者：
WEIDNER, N;FOLKMAN, J;GASPARINI, G
通讯作者：
GASPARINI, G
影响因子：
3.7
作者：
Junnila, Siina;Kokkola, Arto;Monni, Outi
通讯作者：
Monni, Outi