Alginate inhibits iron absorption from ferrous gluconate in a randomized controlled trial and reduces iron uptake into Caco-2 cells.
Alginate inhibits iron absorption from ferrous gluconate in a randomized controlled trial and reduces iron uptake into Caco-2 cells.
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DOI：
10.1371/journal.pone.0112144
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Fairweather-Tait SJ
中科院分区：
文献类型：
--
作者：
Wawer AA;Harvey LJ;Dainty JR;Perez-Moral N;Sharp P;Fairweather-Tait SJ
先前的体外实验结果表明，海藻酸盐珠可能是一种有用的食品铁强化载体。进行了一项人体研究，以检验藻酸盐增强铁吸收的假设。进行了一项随机、单盲、交叉试验，根据试验餐后血清铁的出现情况来测量铁的吸收。隔夜禁食的志愿者(n = 15)被给予200g可乐味果冻和21毫克铁作为葡萄糖酸亚铁的测试餐，要么是混合在果冻中的海藻酸珠，要么是胶囊中的。藻酸盐微珠的铁吸收率低于葡萄糖酸亚铁(分别为8.5%和12.6%，p = 0.003)。B组(n = 9)同时进食试验餐和钙6 0 0 mg，以确定海藻酸盐是否改变了钙的抑制作用。钙使葡萄糖酸亚铁的铁吸收减少了51%，从11.5%减少到5.6%(p = 0.014)，从海藻酸珠减少了37%，从8.3%减少到5.2%(p = 0.009)。利用Caco-2细胞进行体外实验，探讨了先前体外实验结果与人体实验结果不同的原因；证实了海藻酸盐的抑制作用。与人类研究中使用的珠子类似，在含有和不含有可乐果冻的情况下，进行模拟胃肠道消化，并将消化作用应用于Caco-2细胞。藻酸盐和可乐果冻均显著降低细胞铁摄取率，分别为34%(p = 0.009)和35%(p = 0.003)。可乐果冻和钙的组合产生了非常低的铁蛋白反应，只有单独使用葡萄糖酸亚铁的16.5%(p<；0.001)。这些研究的结果表明，海藻酸盐珠不是用于食品强化的可溶性铁盐的有效输送系统。ClinicalTrials.gov NCT01528644
Previous in vitro results indicated that alginate beads might be a useful vehicle for food iron fortification. A human study was undertaken to test the hypothesis that alginate enhances iron absorption. A randomised, single blinded, cross-over trial was carried out in which iron absorption was measured from serum iron appearance after a test meal. Overnight-fasted volunteers (n = 15) were given a test meal of 200 g cola-flavoured jelly plus 21 mg iron as ferrous gluconate, either in alginate beads mixed into the jelly or in a capsule. Iron absorption was lower from the alginate beads than from ferrous gluconate (8.5% and 12.6% respectively, p = 0.003). Sub-group B (n = 9) consumed the test meals together with 600 mg calcium to determine whether alginate modified the inhibitory effect of calcium. Calcium reduced iron absorption from ferrous gluconate by 51%, from 11.5% to 5.6% (p = 0.014), and from alginate beads by 37%, from 8.3% to 5.2% (p = 0.009). In vitro studies using Caco-2 cells were designed to explore the reasons for the difference between the previous in vitro findings and the human study; confirmed the inhibitory effect of alginate. Beads similar to those used in the human study were subjected to simulated gastrointestinal digestion, with and without cola jelly, and the digestate applied to Caco-2 cells. Both alginate and cola jelly significantly reduced iron uptake into the cells, by 34% (p = 0.009) and 35% (p = 0.003) respectively. The combination of cola jelly and calcium produced a very low ferritin response, 16.5% (p<0.001) of that observed with ferrous gluconate alone. The results of these studies demonstrate that alginate beads are not a useful delivery system for soluble salts of iron for the purpose of food fortification. ClinicalTrials.gov NCT01528644
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影响因子：
7.1
作者：
Minihane, AM;Fairweather-Tait, SJ
通讯作者：
Fairweather-Tait, SJ
影响因子：
7.1
作者：
COOK, JD;MONSEN, ER
通讯作者：
MONSEN, ER
DOI：
10.1024/0300-9831/a000036
发表时间：
2010-10-01
影响因子：
2.3
作者：
Loennerdal, Bo
通讯作者：
Loennerdal, Bo
影响因子：
5.8
作者：
Chan, LW;Jin, Y;Heng, PWS
通讯作者：
Heng, PWS
影响因子：
4.4
作者：
Bosscher, D;Van Caillie-Bertrand, M;Deelstra, H
通讯作者：
Deelstra, H