Transcriptional response of the calcification and stress response toolkits in an octocoral under heat and pH stress

Transcriptional response of the calcification and stress response toolkits in an octocoral under heat and pH stress
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热和 pH 胁迫下八珊瑚钙化和应激反应工具包的转录反应

DOI:
10.1101/2020.07.15.202069
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发表时间:
2020
期刊:
bioRxiv
影响因子:
--
通讯作者:
Wörheide
Wörheide
中科院分区:
--
文献类型:
--
作者:
Vargas;Zimmer;Lehmann;Wörheide

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多达三分之一的海洋物种栖息在珊瑚礁中,但由于过度捕捞、富营养化、海洋变暖和酸化等当地和全球威胁,这些高度多样化的生态系统的未来是不安全的。虽然预计这些影响将对珊瑚礁产生净有害影响,但已经表明,珊瑚礁等一些生物可能不受影响,或受益于生物学引起的环境变化,并可能在未来的珊瑚礁中取代石珊瑚。尽管它们在未来的浅水沿海环境中具有潜在的重要性,但导致珊瑚中观察到的生物诱导应力恢复力的分子机制仍然未知。在这里,我们使用操纵实验,蛋白质组学和转录组学来证明,一种常见的印度太平洋柳珊瑚珊瑚Pinnigorgia flava用于存款其碳酸钙骨架的分子工具包对热和海水酸化应激具有弹性。亚致死热胁迫引发黄胸滨藜的应激反应,但不影响编码骨架有机基质(SOM)蛋白的27个转录本的表达。暴露于海水酸化并没有引起应激反应,但引发了许多转录下调,包括在SOM中存在的成骨细胞同源物。所观察到的骨骼生成和应激反应工具包的转录解耦提供了对在珊瑚中观察到的由遗传驱动的环境变化的恢复机制的见解。
Up to one‐third of all described marine species inhabit coral reefs, but the future of these hyperdiverse ecosystems is insecure due to local and global threats, such as overfishing, eutrophication, ocean warming and acidification. Although these impacts are expected to have a net detrimental effect on reefs, it has been shown that some organisms such as octocorals may remain unaffected, or benefit from, anthropogenically induced environmental change, and may replace stony corals in future reefs. Despite their potential importance in future shallow‐water coastal environments, the molecular mechanisms leading to the resilience to anthropogenically induced stress observed in octocorals remain unknown. Here, we use manipulative experiments, proteomics and transcriptomics to show that the molecular toolkit used byPinnigorgia flava, a common Indo‐Pacific gorgonian octocoral, to deposit its calcium carbonate skeleton is resilient to heat and seawater acidification stress. Sublethal heat stress triggered a stress response inP.flavabut did not affect the expression of 27 transcripts encoding skeletal organic matrix (SOM) proteins. Exposure to seawater acidification did not cause a stress response but triggered the downregulation of many transcripts, including an osteonidogen homologue present in the SOM. The observed transcriptional decoupling of the skeletogenic and stress‐response toolkits provides insights into the mechanisms of resilience to anthropogenically driven environmental change observed in octocorals.
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