Comparative Proteomics of Octocoral and Scleractinian Skeletomes and the Evolution of Coral Calcification.
Comparative Proteomics of Octocoral and Scleractinian Skeletomes and the Evolution of Coral Calcification.
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DOI：
10.1093/gbe/evaa162
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发表时间：
2020-09-01
影响因子：
3.3
通讯作者：
Wörheide G
中科院分区：
文献类型：
--
作者：
Conci N;Lehmann M;Vargas S;Wörheide G
珊瑚是珊瑚礁的生态系统工程师，珊瑚礁是生物多样性最丰富的海洋生态系统之一。珊瑚形成珊瑚礁的能力取决于生物控制下碳酸钙（CaCO 3）的沉淀。然而，珊瑚生物矿化的几种机制仍然难以捉摸，例如，珊瑚是否采用不同的分子机制来存款不同的CaCO 3多晶型物（即，文石或方解石）。在这里，我们使用串联质谱（MS/MS）比较蛋白质闭塞在骨骼的三个珊瑚和一个scleractinian物种：Tubipora musica和Sinularia cf。cruciata（方解石片晶）、蓝珊瑚Heliopora coerulea（文石骨架）和石珊瑚文石Montipora digitata。相互爆炸分析发现文石和方解石物种之间的重叠非常低，而一套核心的蛋白质是共同的珊瑚生产方解石片。然而，碳酸酐酶CruCA 4存在于两种多晶型物的骨架中。系统发育分析强调了珊瑚中蛋白质共存的几个可能的例子。这些包括酸性蛋白质和硬化素，它们似乎是钙化的次要补充，可能来自发挥不同功能的蛋白质。珊瑚和硬珊瑚之间的相似之处包括存在一种半乳糖苷相关蛋白、碳酸酐酶和一种类肝素蛋白。虽然前两人似乎是独立招募的，但第三人似乎有共同的出身。这项工作是首次尝试识别和比较与珊瑚骨架多晶型多样性相关的蛋白质，提供了几个新的研究目标，并使未来的功能和进化研究，旨在阐明珊瑚生物矿化的起源和进化。
Corals are the ecosystem engineers of coral reefs, one of the most biodiverse marine ecosystems. The ability of corals to form reefs depends on the precipitation of calcium carbonate (CaCO3) under biological control. However, several mechanisms underlying coral biomineralization remain elusive, for example, whether corals employ different molecular machineries to deposit different CaCO3 polymorphs (i.e., aragonite or calcite). Here, we used tandem mass spectrometry (MS/MS) to compare the proteins occluded in the skeleton of three octocoral and one scleractinian species: Tubipora musica and Sinularia cf. cruciata (calcite sclerites), the blue coral Heliopora coerulea (aragonitic skeleton), and the scleractinian aragonitic Montipora digitata. Reciprocal Blast analysis revealed extremely low overlap between aragonitic and calcitic species, while a core set of proteins is shared between octocorals producing calcite sclerites. However, the carbonic anhydrase CruCA4 is present in the skeletons of both polymorphs. Phylogenetic analysis highlighted several possible instances of protein co-option in octocorals. These include acidic proteins and scleritin, which appear to have been secondarily recruited for calcification and likely derive from proteins playing different functions. Similarities between octocorals and scleractinians included presence of a galaxin-related protein, carbonic anhydrases, and one hephaestin-like protein. Although the first two appear to have been independently recruited, the third appear to share a common origin. This work represents the first attempt to identify and compare proteins associated with coral skeleton polymorph diversity, providing several new research targets and enabling both future functional and evolutionary studies aimed at elucidating the origin and evolution of coral biomineralization.
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影响因子：
3.8
作者：
Amos, Fairland F.;Destine, Edly;Evans, John Spencer
通讯作者：
Evans, John Spencer
影响因子：
3.3
作者：
Conci, Nicola;Woerheide, Gert;Vargas, Sergio
通讯作者：
Vargas, Sergio
DOI：
10.1074/jbc.m112.352005
发表时间：
2012-06-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Debreuil J;Tambutté E;Zoccola D;Deleury E;Guigonis JM;Samson M;Allemand D;Tambutté S
通讯作者：
Tambutté S
DOI：
10.1016/j.cbpb.2008.10.003
发表时间：
2009-02-01
影响因子：
2.2
作者：
Farre, B.;Dauphin, Y.
通讯作者：
Dauphin, Y.
影响因子：
1.4
作者：
Flores, Rachel L.;Gonzales, Kayla;Livingston, Brian T.
通讯作者：
Livingston, Brian T.