Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia.
Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia.
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DOI：
10.1186/s12936-022-04250-2
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发表时间：
2022-08-01
期刊：
影响因子：
3
通讯作者：
Golassa, Lemu
中科院分区：
文献类型：
--
作者：
Abate, Andargie;Bouyssou, Isabelle;Mabilotte, Solenne;Doderer-Lang, Cecile;Dembele, Laurent;Menard, Didier;Golassa, Lemu
在非洲达菲阴性个体中检测到间日疟原虫感染的增加挑战了建立间日疟原虫裂殖子用于侵入网织红细胞的独特间日疟原虫达菲结合蛋白-达菲趋化因子抗原受体（PvDBP-DARC）途径的教条。关于达菲抗原多态性对间日疟流行病学影响的信息仍然难以捉摸。本研究的目的是根据Duffy抗原多态性来确定埃塞俄比亚间日疟原虫无性寄生虫病的分布。从健康中心前瞻性招募的138例间日疟原虫感染患者中收集的干血斑（DBS）中提取DNA。通过显微镜检查和实时定量聚合酶链反应（PCR）进行间日疟原虫无性寄生虫病的鉴定和估计。在总共138份间日疟原虫感染的样品中通过DNA测序进行Duffy基因分型。间日疟原虫感染者中Duffy阴性（FY*BES/FY*BES）占2.9%（4/138）。Duffy基因型FY*B/FY*BES最常见（48.6%），其次为FY*A/FY*BES基因型（25.4%）。在一名患者中，观察到FY*02 W.01/FY*02 N.01基因型赋予Fyb抗原的弱表达。所有感染间日疟原虫的Duffy阴性患者均表现出较低的无性寄生虫血症（≤ 110个寄生虫/µL）。Duffy阴性患者的中位间日疟原虫寄生虫血症（53个寄生虫/µL）显著低于纯合子和杂合子个体（P < 0.0001）。Duffy阴性患者的间日疟原虫无一例外地表现出低无性寄生虫血症。这一发现表明，间日疟原虫用于侵入Duffy阴性网织红细胞的途径比Duffy阳性网织红细胞中使用的途径效率低得多。此外，在达菲阴性个体中观察到的低无性寄生虫血症可能构成“未被发现的沉默的寄生虫”，从而可能推迟埃塞俄比亚间日疟的消除。在线版本包含补充材料，可通过10.1186/s12936-022-04250-2获得。
The increase in detections of Plasmodium vivax infection in Duffy-negative individuals in Africa has challenged the dogma establishing the unique P. vivax Duffy Binding Protein-Duffy antigen receptor for chemokines (PvDBP-DARC) pathway used by P. vivax merozoites to invade reticulocytes. Information on the impact of Duffy antigen polymorphisms on the epidemiology of P. vivax malaria remains elusive. The objective of this study was to determine the distribution of asexual parasitaemia of P. vivax according to the Duffy antigen polymorphisms in Ethiopia. DNA was extracted from dried blood spots (DBS) collected from prospectively recruited 138 P. vivax-infected patients from health centres. The identification and estimation of P. vivax asexual parasitaemia were performed by microscopic examination and quantitative real-time polymerase chain reaction (PCR). Duffy genotyping was conducted by DNA sequencing in a total of 138 P.vivax infected samples. The proportion of Duffy-negatives (FY*BES/FY*BES) in P. vivax infected patients was 2.9% (4/138). Duffy genotype FY*B/FY*BES (48.6%) was the most common, followed by FY*A/FY*BES genotype (25.4%). In one patient, the FY*02 W.01/FY*02 N.01 genotype conferring a weak expression of the Fyb antigen was observed. All P.vivax infected Duffy-negative patients showed low asexual parasitaemia (≤ 110 parasites/µL). The median P. vivax parasitaemia in Duffy-negative patients (53 parasites/µL) was significantly lower than those found in homozygous and heterozygous individuals (P < 0.0001). Plasmodium vivax in Duffy-negative patients shows invariably low asexual parasitaemia. This finding suggests that the pathway used by P. vivax to invade Duffy-negative reticulocytes is much less efficient than that used in Duffy-positives. Moreover, the low asexual parasitaemia observed in Duffy-negative individuals could constitute an ‘undetected silent reservoir', thus likely delaying the elimination of vivax malaria in Ethiopia. The online version contains supplementary material available at 10.1186/s12936-022-04250-2.
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影响因子：
8.4
作者：
Lo, Eugenia;Russo, Gianluca;Pestana, Kareen;Kepple, Daniel;Abagero, Beka Raya;Dongho, Ghyslaine Bruna Djeunang;Gunalan, Karthigayan;Miller, Louis H.;Hamid, Muzamil Mahdi Abdel;Yewhalaw, Delenasaw;Paganotti, Giacomo Maria
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作者：
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影响因子：
16.6
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