Molecular Characterization of Respiratory Syncytial Virus in Children With Repeated Infections With Subgroup B in the Philippines.
Molecular Characterization of Respiratory Syncytial Virus in Children With Repeated Infections With Subgroup B in the Philippines.
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DOI：
10.1093/infdis/jiy256
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发表时间：
2018-08-24
期刊：
影响因子：
--
通讯作者：
Oshitani H
中科院分区：
文献类型：
--
作者：
Okamoto M;Dapat CP;Sandagon AMD;Batangan-Nacion LP;Lirio IC;Tamaki R;Saito M;Saito-Obata M;Lupisan SP;Oshitani H
人呼吸道合胞病毒（RSV）是婴幼儿严重急性呼吸道感染的主要病原，以反复感染为特征。然而，氨基酸替换在反复感染中的作用仍不清楚。因此，本研究的目的是阐明RSV在反复感染的儿童中的遗传特征，使用F和G基因的分子分析。我们对菲律宾5岁以下的儿童进行了一项队列研究。我们收集了急性呼吸道症状儿童的鼻咽拭子，并比较了初始和后续RSV感染之间的F和G序列。我们从2014年5月至2016年1月检查了1802名儿童，收集了3471份样本。在25名儿童中观察到重复感染，包括4名同源RSV-B再感染。来自4对同源再感染的病毒在G蛋白中主要在O-糖基化位点发生氨基酸替换，而在F蛋白中的变化在抗原位点V（L173 S）和θ（Q209 K）处被鉴定，这些抗原位点被认为是F蛋白融合前构象的必需表位。RSV-B的G和F蛋白中的氨基酸取代可能导致抗原性变化，可能导致本研究中观察到的同源再感染。RSV-B F蛋白抗原位点θ的Q209 K取代和抗原位点V的L173 S取代以及G蛋白O-连接糖基化模式的变化可能导致儿童同源再感染。
Human respiratory syncytial virus (RSV) is the leading cause of severe acute respiratory infection in infants and young children, which is characterized by repeated infections. However, the role of amino acid substitutions in repeated infections remains unclear. Hence, this study aimed to elucidate the genetic characteristics of RSV in children with repeated infections using molecular analyses of F and G genes. We conducted a cohort study of children younger than 5 years in the Philippines. We collected nasopharyngeal swabs from children with acute respiratory symptoms and compared F and G sequences between initial and subsequent RSV infections. We examined 1802 children from May 2014 to January 2016 and collected 3471 samples. Repeated infections were observed in 25 children, including 4 with homologous RSV-B reinfections. Viruses from the 4 pairs of homologous reinfections had amino acid substitutions in the G protein mostly at O-glycosylation sites, whereas changes in the F protein were identified at antigenic sites V (L173S) and θ (Q209K), considered essential epitopes for the prefusion conformation of the F protein. Amino acid substitutions in G and F proteins of RSV-B might have led to antigenic changes, potentially contributing to homologous reinfections observed in this study. The Q209K substitution in antigenic site θ and L173S substitution in antigenic site V of F protein and changes in O-linked glycosylation pattern in G protein of RSV-B may contribute to the homologous reinfections in children.
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影响因子：
3.7
作者：
Eshaghi A;Duvvuri VR;Lai R;Nadarajah JT;Li A;Patel SN;Low DE;Gubbay JB
通讯作者：
Gubbay JB
影响因子：
1.6
作者：
Agenbach, E;Tiemessen, CT;Venter, M
通讯作者：
Venter, M
DOI：
10.1126/science.1234914
发表时间：
2013-05-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
McLellan JS;Chen M;Leung S;Graepel KW;Du X;Yang Y;Zhou T;Baxa U;Yasuda E;Beaumont T;Kumar A;Modjarrad K;Zheng Z;Zhao M;Xia N;Kwong PD;Graham BS
通讯作者：
Graham BS
影响因子：
6.4
作者：
MUELENAER, PM;HENDERSON, FW;MURPHY, BR
通讯作者：
MURPHY, BR
影响因子：
6.4
作者：
HALL, CB;WALSH, EE;SCHNABEL, KC
通讯作者：
SCHNABEL, KC