Display of epitopes on the surface of tobacco mosaic virus: impact of charge and isoelectric point of the epitope on virus-host interactions.
Display of epitopes on the surface of tobacco mosaic virus: impact of charge and isoelectric point of the epitope on virus-host interactions.
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DOI：
10.1006/jmbi.1999.2860
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发表时间：
1999-07-02
影响因子：
5.6
通讯作者：
Beachy RN
中科院分区：
文献类型：
--
作者：
Bendahmane M;Koo M;Karrer E;Beachy RN
The biophysical properties of the tobacco mosaic tobamovirus (TMV) coat protein (CP) make it possible to display foreign peptides on the surface of TMV. The immunogenic epitopes G5-24 from the rabies virus (RV) glycoprotein, and 5B19 from murine hepatitis virus (MHV) S-glycoprotein were successfully displayed on the surface of TMV, and viruses accumulated to high levels in infected leaves of Nicotiana tabacum Xanthi-nn. The peptide RB19, which contains an arginine residue plus the 5B19 epitope fused to the CP (TMV-RB19), resulted in the induction of necrotic local lesions on inoculated leaves of N. tabacum Xanthi-nn and cell death of infected BY2 protoplasts. RNA dot blot assays confirmed that expression of the acidic and basic pathogenesis-related PR2 genes were induced in infected Xanthi-nn leaf tissue. TMV that carried epitope 31D from the RV nucleoprotein did not accumulate in inoculated tobacco leaves. Analysis of hybrid CPs predicted that the isoelectric points (pI):charge value was 5.31: − 2 for wild-type CP, 5.64: − 1 for CP-RB19, and 9.14: + 2 for CP-31D. When acidic amino acids were inserted in CP-RB19 and CP-31D to bring their pI:charge to near that of wild-type CP, the resulting viruses TMV-RB19E and TMV-4D:31D infected N. tabacum Xanthi-nn plants and BY2 protoplasts without causing cell death. These data show the importance of the pI of the epitope and its effects on the hybrid CP pI:charge value for successful epitope display as well as the lack of tolerance to positively charged epitopes on the surface of TMV.
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影响因子：
5.4
作者：
Bendahmane, M;Fitchen, JH;Beachy, RN
通讯作者：
Beachy, RN
影响因子：
5.5
作者：
FITCHEN, J;BEACHY, RN;HEIN, MB
通讯作者：
HEIN, MB
影响因子：
3.5
作者：
HEITZ, T;FRITIG, B;LEGRAND, M
通讯作者：
LEGRAND, M
影响因子：
5.1
作者：
Karrer, EE;Beachy, RN;Holt, CA
通讯作者：
Holt, CA
DOI：
10.1073/pnas.79.19.5818
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GOELET, P;LOMONOSSOFF, GP;KARN, J
通讯作者：
KARN, J