Study on activity of the signaling pathways regulating hepatocyte differentiation during rat liver regeneration
Study on activity of the signaling pathways regulating hepatocyte differentiation during rat liver regeneration
复制标题
大鼠肝脏再生过程中调控肝细胞分化的信号通路活性研究
DOI：
10.1080/19768354.2014.982707
复制
发表时间：
2014-11
影响因子：
2.9
通讯作者：
Cunshuan Xu
中科院分区：
文献类型：
--
作者：
Jing Yang;Menghua Li;Yun Zhou;Cunshuan Xu
为了从基因转录水平了解大鼠肝脏再生（LR）过程中肝细胞分化的机制，采用Rat Genome 230 2.0 Array测定肝细胞基因表达变化。然后通过NCBI、QIAGEN和KEGG网络搜索与细胞分化相关的基因及其信号通路，并根据表达谱通过谱函数Ep(t)计算其信号活性。采用同类提取方法对与信号通路和细胞分化相关的关键基因进行了分析和验证。采用Ingenuity Pathway Analysis 9.0 (IPA)分析大鼠LR过程中肝细胞分化的机制。结果显示，42条信号通路调控细胞分化，IL-9信号通路及其JAK/STAT、PI3K/AKT分支、PEDF信号通路及其NF-κB、Ras/ERK分支的Ep(t)值在大鼠LR进展期(部分肝切除[PH]后6~72 h)显着升高，肝细胞分化的Ep(t)值也显着升高。 ERK/MAPK信号通路在启动期（PH后0.5-6小时）和进展期显着增加，肝细胞分化也显着增加。总之，在调节细胞分化的42条信号通路中，IL-9信号通路及其JAK/STAT和PI3K/AKT分支的信号活性、PEDF信号通路及其NF-κB和Ras/ERK分支、ERK/MAPK信号活性显着增强，并促进大鼠LR的肝细胞分化。同时，参与上述信号通路的MYC表达以及与细胞分化相关的AGT、BCL2、IL10、CCND1、PLAT和NF1的表达显着增加。
To understand the mechanism of hepatocyte differentiation during rat liver regeneration (LR) at the gene transcription level, Rat Genome 230 2.0 Array was used to determine the gene expression changes of hepatocytes. Then the genes associated with cell differentiation and their signaling pathways were searched by NCBI, QIAGEN and KEGG webs, and their signaling activities were calculated by spectral function Ep(t) based on the expression profiles. The key genes associated with the signaling pathways and cell differentiation were analyzed and verified by the same kind extraction method. The mechanism of hepatocyte differentiation during rat LR was analyzed by Ingenuity Pathway Analysis 9.0 (IPA). The results showed that 42 signaling pathways regulated cell differentiation and the Ep(t) values of IL-9 signaling pathway and its JAK/STAT and PI3K/AKT branches, PEDF signaling pathway and its NF-κB and Ras/ERK branches were significantly increased in the progression phase (6–72 h after partial hepatectomy [PH]) of rat LR, and the Ep(t) values of hepatocyte differentiation were also significantly increased. ERK/MAPK signaling pathway was significantly increased in the priming (0.5–6 h after PH) and progression phase, and hepatocyte differentiation were also significantly increased. In conclusion, among the 42 signaling pathways that regulated cell differentiation, the signaling activity of IL-9 signaling pathway and its JAK/STAT and PI3K/AKT branches, PEDF signaling activity and its NF-κB and Ras/ERK branches, and ERK/MAPK signaling activity were significantly increased and promoted hepatocyte differentiation in rat LR. Meanwhile, the expression of MYC involved in mentioned signaling pathways and expression of AGT, BCL2, IL10, CCND1, PLAT, and NF1 related to cell differentiation were increased significantly.
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影响因子：
13.5
作者：
Fausto, N;Campbell, JS;Riehle, KJ
通讯作者：
Riehle, KJ
影响因子：
10.5
作者：
Jixuan Li;Gang Ning;Stephen A. Duncan
通讯作者：
Jixuan Li;Gang Ning;Stephen A. Duncan
DOI：
10.1002/9781119436812.ch45
发表时间：
2020-01
期刊：
The Liver
影响因子：
--
作者：
G. Michalopoulos
通讯作者：
G. Michalopoulos
影响因子：
5.8
作者：
Michalopoulos, George K.
通讯作者：
Michalopoulos, George K.
影响因子：
25.7
作者：
Fausto, N
通讯作者：
Fausto, N