Role of Porphyromonas gingivalis gingipains in multi-species biofilm formation.
Role of Porphyromonas gingivalis gingipains in multi-species biofilm formation.
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DOI：
10.1186/s12866-014-0258-7
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发表时间：
2014-10-02
期刊：
影响因子：
4.2
通讯作者：
Bostanci N
中科院分区：
文献类型：
--
作者：
Bao K;Belibasakis GN;Thurnheer T;Aduse-Opoku J;Curtis MA;Bostanci N
牙周病是一种多菌疾病，会导致牙齿支持(牙周)组织的炎性破坏。它们的发生归因于龈下生物膜的形成，这些生物膜刺激了受影响组织的一连串慢性炎症反应。革兰氏阴性厌氧菌牙龈卟啉单胞菌、连翘坦纳氏菌和齿密螺旋体是龈下生物膜微生物区系的一部分，与疾病的发生和严重程度密切相关。牙龈假单胞菌表达几种毒力因子，这些毒力因子可能支持它的生存，调节它与生物膜中其他物种的沟通，或者调节定植的宿主组织的炎症反应。这些毒力因子中最突出的是牙龈痛，它是一组半胱氨酸蛋白酶(Arg特异性或赖氨酸特异性)。牙周疼痛在牙龈假单胞菌生物膜形成能力中的作用几乎没有被研究过。因此，这项体外研究采用了一个由10种“龈下”细菌组成的生物膜模型，其中包括一株野生型Pggivalis菌株或其衍生的Lys-gigiain和Arg-gigipa等基因突变体，以评估生物膜组成的定量和定性变化。在生物膜生长后，用荧光原位杂交或免疫荧光法对所有10种细菌的水平进行定量。野生型和两株牙龈痛缺陷假单胞菌在其相应的生物膜中显示出相似的生长。在剩下的9个物种中，只有当生物膜中存在Lys-牙周疼痛突变体时，连翘的数量才显著减少。当用激光共聚焦扫描显微镜评价生物膜的结构时，最突出的观察是齿状毛滴虫的空间排列发生了变化，存在牙龈假单胞菌Arg-gigiain突变体。牙龈假单胞菌的牙龈痛可能会定性和定量地影响多菌生物膜的组成。目前的实验模型揭示了牙龈假单胞菌和连翘或齿假单胞菌之间的相互依赖关系。
Periodontal diseases are polymicrobial diseases that cause the inflammatory destruction of the tooth-supporting (periodontal) tissues. Their initiation is attributed to the formation of subgingival biofilms that stimulate a cascade of chronic inflammatory reactions by the affected tissue. The Gram-negative anaerobes Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola are commonly found as part of the microbiota of subgingival biofilms, and they are associated with the occurrence and severity of the disease. P. gingivalis expresses several virulence factors that may support its survival, regulate its communication with other species in the biofilm, or modulate the inflammatory response of the colonized host tissue. The most prominent of these virulence factors are the gingipains, which are a set of cysteine proteinases (either Arg-specific or Lys-specific). The role of gingipains in the biofilm-forming capacity of P. gingivalis is barely investigated. Hence, this in vitro study employed a biofilm model consisting of 10 “subgingival” bacterial species, incorporating either a wild-type P. gingivalis strain or its derivative Lys-gingipain and Arg-gingipan isogenic mutants, in order to evaluate quantitative and qualitative changes in biofilm composition. Following 64 h of biofilm growth, the levels of all 10 species were quantified by fluorescence in situ hybridization or immunofluorescence. The wild-type and the two gingipain-deficient P. gingivalis strains exhibited similar growth in their corresponding biofilms. Among the remaining nine species, only the numbers of T. forsythia were significantly reduced, and only when the Lys-gingipain mutant was present in the biofilm. When evaluating the structure of the biofilm by confocal laser scanning microscopy, the most prominent observation was a shift in the spatial arrangement of T. denticola, in the presence of P. gingivalis Arg-gingipain mutant. The gingipains of P. gingivalis may qualitatively and quantitatively affect composition of polymicrobial biofilms. The present experimental model reveals interdependency between the gingipains of P. gingivalis and T. forsythia or T. denticola.
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DOI：
10.4049/jimmunol.1202339
发表时间：
2012-12-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Abe T;Hosur KB;Hajishengallis E;Reis ES;Ricklin D;Lambris JD;Hajishengallis G
通讯作者：
Hajishengallis G
影响因子：
4.2
作者：
Guggenheim, Bernhard;Gmuer, Rudolf;Kinane, Denis F.
通讯作者：
Kinane, Denis F.
影响因子：
3.2
作者：
BRAMANTI, TE;HOLT, SC
通讯作者：
HOLT, SC
影响因子：
7.6
作者：
Bostanci, N.;Emingil, G.;Belibasakis, G. N.
通讯作者：
Belibasakis, G. N.
影响因子：
3.7
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ