Characterization of Growth and Reproduction Performance, Transgene Integration, Expression, and Transmission Patterns in Transgenic Pigs Produced by piggyBac Transposition-Mediated Gene Transfer.
Characterization of Growth and Reproduction Performance, Transgene Integration, Expression, and Transmission Patterns in Transgenic Pigs Produced by piggyBac Transposition-Mediated Gene Transfer.
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DOI：
10.1080/10495398.2016.1178140
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发表时间：
2016-10
影响因子：
3.7
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Zeng F;Li Z;Cai G;Gao W;Jiang G;Liu D;Urschitz J;Moisyadi S;Wu Z
我们利用一种新开发的基于piggyBac质粒胞质注射的高效、简便的猪转基因方法，成功地获得了一批表达EGFP的创始者转基因猪。在本研究中，我们研究了生长和繁殖性能，并表征了转基因猪的piggyBac转座转基因插入，传输和表达模式。结果表明，转基因对猪的生长和繁殖无不良影响。将多拷贝的单基因EGFP转基因插入到宿主基因组的非编码区，并以分离或连锁的方式从建立者转基因猪传递给其转基因后代。EGFP转基因在转基因猪中广泛表达，其表达强度与转基因拷贝数相关，但与其启动子DNA甲基化水平无关。据我们所知，这是第一个全面描述piggyBac系统生产的转基因猪的生长和繁殖性能，转基因插入，表达和传递谱的研究。这不仅证明了piggyBac转座介导的转基因是一种有效的转基因方法，而且为了解piggyBac转座转基因动物中转基因的插入、表达和传递模式提供了科学依据。
Previously we successfully produced a group of EGFP-expressing founder transgenic pigs by a newly developed efficient and simple pig transgenesis method based on cytoplasmic injection of piggyBac plasmids. In this study, we investigated the growth and reproduction performance, and characterized the transgene insertion, transmission and expression patterns in transgenic pigs generated by piggyBac transposition. Results showed that transgene has no injurious effect on the growth and reproduction of transgenic pigs. Multiple copies of monogenic EGFP transgene were inserted at noncoding sequences of host genome, and passed from founder transgenic pigs to their transgenic offspring in segregation or linkage manner. The EGFP transgene was ubiquitously expressed in transgenic pigs, and its expression intensity was associated with transgene copy number but not related to its promoter DNA methylation level. To the best of our knowledge, this is first study that fully described the growth and reproduction performance, transgene insertion, expression and transmission profiles in transgenic pigs produced by piggyBac system. It not only demonstrates that piggyBac transposition-mediated gene transfer is an effective and favourable approach for pig transgenesis, but also provides scientific information for understanding the transgene insertion, expression and transmission patterns in transgenic animals produced by piggyBac transposition.
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影响因子：
3
作者：
Wu, Zhenfang;Xu, Zhiqian;Zou, Xian;Zeng, Fang;Shi, Junsong;Liu, Dewu;Urschitz, Johann;Moisyadi, Stefan;Li, Zicong
通讯作者：
Li, Zicong
DOI：
10.1002/(sici)1098-2795(200006)56:2
发表时间：
2000-06-01
影响因子：
2.5
作者：
Balhorn, R;Brewer, L;Corzett, M
通讯作者：
Corzett, M
影响因子：
56.9
作者：
Perry, ACF;Wakayama, T;Yanagimachi, R
通讯作者：
Yanagimachi, R
影响因子：
3.6
作者：
Li, Zicong;Zeng, Fang;Wu, Zhenfang
通讯作者：
Wu, Zhenfang
影响因子：
3.7
作者：
Kong Q;Wu M;Huan Y;Zhang L;Liu H;Bou G;Luo Y;Mu Y;Liu Z
通讯作者：
Liu Z