A genetics-based approach confirms immune associations with life history across multiple populations of an aquatic vertebrate (Gasterosteus aculeatus).
A genetics-based approach confirms immune associations with life history across multiple populations of an aquatic vertebrate (Gasterosteus aculeatus).
复制标题
一种基于遗传学的方法证实了在水生脊椎动物（Gasterosteus aculeatus）多种种群中与生命史的免疫相关性。
DOI：
10.1111/mec.14772
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发表时间：
2018-08
影响因子：
4.9
通讯作者：
MacColl ADC
中科院分区：
文献类型：
--
作者：
Whiting JR;Magalhaes IS;Singkam AR;Robertson S;D'Agostino D;Bradley JE;MacColl ADC
了解野生免疫变异如何与其他性状协变，可以揭示成本和权衡如何在野外塑造免疫进化。不同的生活史策略可能会增加或减轻免疫成本，有助于以一致的、可测试的方式塑造免疫变异。相反的假设表明，较短的生命史可能会减轻成本抵消他们增加死亡率，或增加成本的影响，如果免疫反应是对发展或生殖权衡。我们研究了三刺棘鱼的岛屿辐射内生活史和免疫反应之间的进化关系，具有不同生活史和寄生的离散种群。我们使用qPCR对两个短寿命群体、两个长寿命群体和一个溯河产卵群体进行采样，以量化当前的免疫特征，并使用RAD‐seq数据研究免疫变体在我们的检测基因内和整个基因组中的分布。短寿命群体表现出所有检测基因的表达显著增加，这与局部等位基因的群体水平变异和可能参与补体途径的基因趋异密切相关。此外，溯河产卵鱼类eda基因的分化可能与炎症增加有关。对全岛15个种群的更广泛分析显示，整个基因组中的免疫基因显示出与生活史策略一起分化的证据。寄生和生殖投资也是表达变异的重要来源，这突出了在野外测定免疫反应时需要谨慎。这些结果为目前的假设提供了强有力的基于基因的支持，这些假设将脊椎动物模型的多个群体中的生活史和免疫变异联系起来。
Understanding how wild immune variation covaries with other traits can reveal how costs and trade‐offs shape immune evolution in the wild. Divergent life history strategies may increase or alleviate immune costs, helping shape immune variation in a consistent, testable way. Contrasting hypotheses suggest that shorter life histories may alleviate costs by offsetting them against increased mortality, or increase the effect of costs if immune responses are traded off against development or reproduction. We investigated the evolutionary relationship between life history and immune responses within an island radiation of three‐spined stickleback, with discrete populations of varying life histories and parasitism. We sampled two short‐lived, two long‐lived and an anadromous population using qPCR to quantify current immune profile and RAD‐seq data to study the distribution of immune variants within our assay genes and across the genome. Short‐lived populations exhibited significantly increased expression of all assay genes, which was accompanied by a strong association with population‐level variation in local alleles and divergence in a gene that may be involved in complement pathways. In addition, divergence around the eda gene in anadromous fish is likely associated with increased inflammation. A wider analysis of 15 populations across the island revealed that immune genes across the genome show evidence of having diverged alongside life history strategies. Parasitism and reproductive investment were also important sources of variation for expression, highlighting the caution required when assaying immune responses in the wild. These results provide strong, gene‐based support for current hypotheses linking life history and immune variation across multiple populations of a vertebrate model.
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影响因子：
4.6
作者：
Dhinaut J;Balourdet A;Teixeira M;Chogne M;Moret Y
通讯作者：
Moret Y
影响因子：
14.9
作者：
NCBI Resource Coordinators
通讯作者：
NCBI Resource Coordinators
影响因子：
5.2
作者：
de Roij, Job;Harris, Philip D.;MacColl, Andrew D. C.
通讯作者：
MacColl, Andrew D. C.
影响因子：
7.7
作者：
Excoffier, Laurent;Lischer, Heidi E. L.
通讯作者：
Lischer, Heidi E. L.
影响因子：
2.6
作者：
Downs, C. J.;Adelman, J. S.;Demas, G. E.
通讯作者：
Demas, G. E.