Water-splitting-based, sustainable and efficient H(2) production in green algae as achieved by substrate limitation of the Calvin-Benson-Bassham cycle.
Water-splitting-based, sustainable and efficient H(2) production in green algae as achieved by substrate limitation of the Calvin-Benson-Bassham cycle.
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DOI：
10.1186/s13068-018-1069-0
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发表时间：
2018
影响因子：
6.3
通讯作者：
Tóth SZ
中科院分区：
文献类型：
--
作者：
Nagy V;Podmaniczki A;Vidal-Meireles A;Tengölics R;Kovács L;Rákhely G;Scoma A;Tóth SZ
光生物制氢具有成为无碳可再生能源的潜力，因为在H2燃烧时，仅产生水。绿色藻类的[Fe-Fe]-型氢化酶是高度活性的，尽管对O2极其敏感。缺硫是诱导H2产生的常见方式，然而，其基本上依赖于有机底物并施加严重的胁迫效应，导致光合机构的降解。我们报告建立一个替代的H2生产方法的绿色藻类，是基于一个短的厌氧诱导，保持卡尔文-本森-Bassham循环不活跃的底物限制和保存氢化酶活性，通过应用一个简单的催化剂，以消除演变的O2。培养物在几天内保持光合活性，电子喂养主要来自水的氢化酶。产生的H2的量高于硫剥夺程序，并且该过程是光自养的。我们的方案表明，可以可持续地使用藻类细胞作为H2生产的全细胞催化剂，这使得藻类生物制氢的工业应用成为可能。本文的在线版本（10.1186/s13068-018-1069-0）包含补充材料，可供授权用户使用。
Photobiological H2 production has the potential of becoming a carbon-free renewable energy source, because upon the combustion of H2, only water is produced. The [Fe–Fe]-type hydrogenases of green algae are highly active, although extremely O2-sensitive. Sulphur deprivation is a common way to induce H2 production, which, however, relies substantially on organic substrates and imposes a severe stress effect resulting in the degradation of the photosynthetic apparatus. We report on the establishment of an alternative H2 production method by green algae that is based on a short anaerobic induction, keeping the Calvin–Benson–Bassham cycle inactive by substrate limitation and preserving hydrogenase activity by applying a simple catalyst to remove the evolved O2. Cultures remain photosynthetically active for several days, with the electrons feeding the hydrogenases mostly derived from water. The amount of H2 produced is higher as compared to the sulphur-deprivation procedure and the process is photoautotrophic. Our protocol demonstrates that it is possible to sustainably use algal cells as whole-cell catalysts for H2 production, which enables industrial application of algal biohydrogen production. The online version of this article (10.1186/s13068-018-1069-0) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Dubini, Alexandra;Ghirardi, Maria L.
通讯作者：
Ghirardi, Maria L.
影响因子：
11.6
作者：
Allorent, Guillaume;Tokutsu, Ryutaro;Finazzi, Giovanni
通讯作者：
Finazzi, Giovanni
影响因子：
2.6
作者：
Braga, Lilian R.;Sarantopoulos, Claire I. G. L.;Braga, Jez W. B.
通讯作者：
Braga, Jez W. B.
影响因子：
7.2
作者：
Batyrova, Khorcheska A.;Tsygankov, Anatoly A.;Kosourov, Sergey N.
通讯作者：
Kosourov, Sergey N.
影响因子：
3
作者：
Ghirardi, ML;Togasaki, RK;Seibert, M
通讯作者：
Seibert, M