Intraepithelial lymphocytes from villus tip and crypt portions of the murine small intestine show distinct characteristics.
Intraepithelial lymphocytes from villus tip and crypt portions of the murine small intestine show distinct characteristics.
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来自小鼠小肠绒毛尖端和隐窝部分的上皮内淋巴细胞显示出独特的特征。
DOI：
10.1016/s0016-5085(98)70258-6
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发表时间：
1998
期刊：
影响因子：
29.4
通讯作者：
Kiyono,H
中科院分区：
文献类型：
--
作者：
Kawabata,S;Boyaka,PN;Coste,M;Fujihashi,K;Yamamoto,M;McGhee,JR;Kiyono,H
背景与目的上皮内淋巴细胞（Intraepithelial lymphocytes，IELs）位于上皮细胞之间，被认为在小肠绒毛尖端和隐窝水平显示独特的特征和功能。方法从小鼠小肠绒毛尖端和隐窝分离IELs，比较IELs自发性细胞因子产生和对白细胞介素（IL）-2和/或IL-7的反应性。结果在绒毛尖端和隐窝中，αβ IEL的数量无差异，而在绒毛尖中，观察到携带γδ型T细胞受体的IEL频率增加的趋势。有趣的是，与绒毛尖相比，腺窝细胞中产生干扰素γ和IL-5的αβ IEL的数量显著减少。此外，绒毛尖端αβ IEL对IL-2和/或IL-7提供的刺激信号的反应比它们的隐窝对应物更高。结论小肠绒毛顶端和隐窝中的γδ IEL与上皮细胞之间存在明显的分子串扰。胃肠病学1998;115：866-873
Background & AimsIntraepithelial lymphocytes (IELs) are located between epithelial cells that are thought to display unique features and functions at the small intestinal villus tip and crypt levels. We have addressed whether the spatial differences in the intestinal epithelium extend to IELs and subsequent cross-talk between IELs and epithelial cells.MethodsIELs were isolated from villus tip and crypt portions of mouse small intestine and then compared for spontaneous cytokine production and responsiveness to interleukin (IL)-2 and/or IL-7.ResultsNo difference was observed between number of αβ IELs in villus tips and crypts, whereas a trend toward increased frequencies of IELs bearing the γδ form of T-cell receptor was noted in villus tips. Interestingly, the number of αβ IELs producing interferon gamma and IL-5 was significantly reduced in the cells from crypts compared with villus tips. Furthermore, villus tip αβ IELs exhibited higher responses to stimulation signals provided by IL-2 and/or IL-7 than their crypt counterpart. Such functional differences were not observed with γδ IELs from the two intestinal sites.ConclusionsDistinct molecular cross-talk between IELs and epithelial cells occurs in intestinal villus tips and crypts. GASTROENTEROLOGY 1998;115:866-873
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影响因子：
3.1
作者：
Culshaw, RJ;Bancroft, GJ;McDonald, V
通讯作者：
McDonald, V
用针对肠细胞表面成分的单克隆抗体研究肠细胞分化。
影响因子：
2.7
作者：
Quaroni,A;Isselbacher,KJ
通讯作者：
Isselbacher,KJ
一种基于碱性磷酸酶的新型分离方法可以对来自小鼠小肠绒毛尖端和隐窝区域的上皮内淋巴细胞进行表征。
DOI：
10.1006/bbrc.1997.7839
发表时间：
1997
期刊：
Biochemical and biophysical research communications.
影响因子：
--
作者：
Kawabata,S;Boyaka,PN;Coste,M;Fujihashi,K;Hamada,S;McGhee,JR;Kiyono,H
通讯作者：
Kiyono,H
大鼠消化系统中的 II 类 MHC 抗原。
影响因子：
6.4
作者：
B. Steiniger;P. Falk;M. Lohmüller;P. H. van der Meide
通讯作者：
P. H. van der Meide
DOI：
10.1073/pnas.86.15.5923
发表时间：
1989-08-01
影响因子：
11.1
作者：
CHAZEN, GD;PEREIRA, GMB;SHEVACH, EM
通讯作者：
SHEVACH, EM