Serum microRNAs profile from genome-wide serves as a fingerprint for diagnosis of acute myocardial infarction and angina pectoris.
Serum microRNAs profile from genome-wide serves as a fingerprint for diagnosis of acute myocardial infarction and angina pectoris.
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全基因组血清 microRNA 谱可作为诊断急性心肌梗塞和心绞痛的指纹。
DOI：
10.1186/1755-8794-6-16
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发表时间：
2013-05-04
影响因子：
2.7
通讯作者：
Xiang Y
中科院分区：
文献类型：
--
作者：
Li C;Fang Z;Jiang T;Zhang Q;Liu C;Zhang C;Xiang Y
为了从全基因组筛选中鉴定用于诊断急性心肌梗死（AMI）和心绞痛（AP）的miRNAs表达谱，我们研究了AMI和AP患者相对早期血清miRNAs的变化。血清样本取自117名AMI患者、182名AP患者和100名年龄和性别匹配的对照组。通过Solexa测序对miRNA表达进行初步筛选。使用RT-qPCR在个体样品中验证差异表达，样品被安排在两个阶段的选择和验证中。Solexa测序结果表明，与对照组相比，AMI患者血清miRNA显著上调。RT-qPCR分析鉴定了六种血清miRNA（miR-1、miR-134、miR-186、miR-208、miR-223和miR-499）的谱作为AMI生物标志物。miR-208和miR-499在AP患者中升高高于AMI患者。ROC曲线表明，一组6个miRNAs具有很大的潜力，为AMI提供敏感和特异的诊断测试。更特别地，六种miRNA的组在AMI和AP病例之间呈现显著差异。从全基因组血清miRNA表达谱中鉴定的六个miRNA签名可以作为AMI和AP诊断的指纹。
In order to identify miRNAs expression profiling from genome-wide screen for diagnosis of acute myocardial infarction (AMI) and angina pectoris (AP), we investigated the altered profile of serum microRNAs in AMI and AP patients at a relative early stage. Serum samples were taken from 117 AMI patients, 182 AP patients and 100 age-and gender-matched controls. An initial screening of miRNAs expression was performed by Solexa sequencing. Differential expression was validated using RT-qPCR in individuals samples, the samples were arranged in a two-phase selection and validation. The Solexa sequencing results demonstrated marked upregulation of serum miRNAs in AMI patients compared with controls. RT-qPCR analysis identified a profile of six serum miRNAs (miR-1, miR-134, miR-186, miR-208, miR-223 and miR-499) as AMI biomarkers. MiR-208 and miR-499 were elevated higher in AP cases than in AMI cases. The ROC curves indicated a panel of six miRNAs has a great potential to offer sensitive and specific diagnostic tests for AMI. More especially, the panel of six miRNAs presents significantly differences between the AMI and AP cases. The six-miRNAs signature identified from genome-wide serum miRNA expression profiling may serves as a fingerprint for AMI and AP diagnosis.
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影响因子：
37.8
作者：
Adams, RJ;Antman, EM;Kavey, REW
通讯作者：
Kavey, REW
影响因子：
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影响因子：
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影响因子：
37.8
作者：
DEWINTER, RJ;KOSTER, RW;SANDERS, GT
通讯作者：
SANDERS, GT
影响因子：
3.7
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Hunter, Melissa Piper;Ismail, Noura;Zhang, Xiaoli;Aguda, Baltazar D.;Lee, Eun Joo;Yu, Lianbo;Xiao, Tao;Schafer, Jeffrey;Lee, Mei-Ling Ting;Schmittgen, Thomas D.;Nana-Sinkam, S. Patrick;Jarjoura, David;Marsh, Clay B.
通讯作者：
Marsh, Clay B.