Maternal patterns of inheritance alter transcript expression in eggs.
Maternal patterns of inheritance alter transcript expression in eggs.
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DOI：
10.1186/s12864-023-09291-8
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发表时间：
2023-04-10
期刊：
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
对早期发育的修改可以导致进化的多样化。发育的早期阶段是由母体控制的，因为母体产生的卵子含有指导早期胚胎发育的营养素、蛋白质和mRNA。母体提供的mRNA是发育初始阶段唯一表达的基因，并且受到严格调控。母体mRNA供应的差异可能导致胚胎发生中的表型变化，并最终导致发育中的进化变化。然而，对于大多数发育模型，卵中母体mRNA表达的变化程度是未知的。在这里，我们使用一个物种的二态发展-女性使鸡蛋和幼虫的不同大小和生活史模式-调查的变化程度在母体mRNA供应的鸡蛋。我们发现，在不同发育模式的卵中，基因表达存在显着差异，并且mRNA表达存在定性和定量差异。我们将亲本效应与等位基因效应分开，发现这两种机制都有助于mRNA表达差异。我们还发现，种内杂交的后代差异提供他们的鸡蛋的基础上，父母的交叉方向（父母的影响），这还没有被证明在生殖性状，如卵子发生。我们发现，不同大小的卵和不同的母体基因型之间，母体控制的发育起始在功能上是不同的。等位基因特异性效应和起源亲本效应都有助于卵中基因表达的差异。后者表明了一种代际效应，即父母的基因型可以影响下一代卵子中的基因表达。在线版本包含补充材料，可通过10.1186/s12864-023-09291-8获得。
Modifications to early development can lead to evolutionary diversification. The early stages of development are under maternal control, as mothers produce eggs loaded with nutrients, proteins and mRNAs that direct early embryogenesis. Maternally provided mRNAs are the only expressed genes in initial stages of development and are tightly regulated. Differences in maternal mRNA provisioning could lead to phenotypic changes in embryogenesis and ultimately evolutionary changes in development. However, the extent that maternal mRNA expression in eggs can vary is unknown for most developmental models. Here, we use a species with dimorphic development— where females make eggs and larvae of different sizes and life-history modes—to investigate the extent of variation in maternal mRNA provisioning to the egg. We find that there is significant variation in gene expression across eggs of different development modes, and that there are both qualitative and quantitative differences in mRNA expression. We separate parental effects from allelic effects, and find that both mechanisms contribute to mRNA expression differences. We also find that offspring of intraspecific crosses differentially provision their eggs based on the parental cross direction (a parental effect), which has not been previously demonstrated in reproductive traits like oogenesis. We find that maternally controlled initiation of development is functionally distinct between eggs of different sizes and maternal genotypes. Both allele-specific effects and parent-of-origin effects contribute to gene expression differences in eggs. The latter indicates an intergenerational effect where a parent’s genotype can affect gene expression in an egg made by the next generation. The online version contains supplementary material available at 10.1186/s12864-023-09291-8.
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影响因子：
64.5
作者：
Ashe A;Sapetschnig A;Weick EM;Mitchell J;Bagijn MP;Cording AC;Doebley AL;Goldstein LD;Lehrbach NJ;Le Pen J;Pintacuda G;Sakaguchi A;Sarkies P;Ahmed S;Miska EA
通讯作者：
Miska EA
影响因子：
2.7
作者：
Celeghin, Andrea;Benato, Francesca;Dalla Valle, Luisa
通讯作者：
Dalla Valle, Luisa
影响因子：
4.4
作者：
Dixon G;Matz M
通讯作者：
Matz M
影响因子：
3
作者：
Duchemin W;Dupont PY;Campbell MA;Ganley AR;Cox MP
通讯作者：
Cox MP
影响因子：
3.3
作者：
Cartwright EL;Lott SE
通讯作者：
Lott SE