Antigen gene and variable number tandem repeat (VNTR) diversity in Theileria parva parasites from Ankole cattle in south-western Uganda: Evidence for conservation in antigen gene sequences combined with extensive polymorphism at VNTR loci.
Antigen gene and variable number tandem repeat (VNTR) diversity in Theileria parva parasites from Ankole cattle in south-western Uganda: Evidence for conservation in antigen gene sequences combined with extensive polymorphism at VNTR loci.
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乌干达西南部 Ankole 牛的细小泰勒虫寄生虫的抗原基因和可变数目串联重复序列 (VNTR) 多样性：抗原基因序列保守性与 VNTR 位点广泛多态性相结合的证据
DOI：
10.1111/tbed.13311
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发表时间：
2020
影响因子：
4.3
通讯作者：
Lubega GW
中科院分区：
文献类型：
--
作者：
Nanteza A;Obara I;Kasaija P;Mwega E;Kabi F;Salih DA;Njahira M;Njuguna J;Odongo D;Bishop RP;Skilton RA;Ahmed J;Clausen PH;Lubega GW
小泰勒虫是一种蜱传播的顶复门原虫寄生虫，感染牛和非洲开普布法罗（Syncerus caffer）的淋巴细胞，在非洲东部、中部和南部引起牛的一种常见致死性疾病。活疫苗接种程序，称为感染和治疗方法（ITM），其最常用的版本包括Muguga、Serengeti转化和基安布5种小锥虫原液，以三价混合物形式递送，通常有效。然而，它并不总是诱导针对异源寄生虫攻击的100%保护。因此，在广泛部署疫苗之前，了解小锥虫目标牛种群的遗传多样性非常重要。本研究使用14个可变数目串联重复序列（VNTR）卫星基因座和两个抗原编码基因（指定为Tp1和Tp2）的序列，研究了来自乌干达西南部Ankole（Bos taurus）牛的T.parvafield分离株的遗传多样性程度，这两个抗原编码基因是ITM诱导的CD8+T细胞应答的靶基因。研究结果显示，T. parvaprevalence为51%，证实了乌干达西南部寄生虫的地方性。牛源性T. parvaVNTR基因型显示出高度多态性。然而，本研究中鉴定的所有thetheT.parvaTp1和Tp2等位基因以前都有报道，表明它们在东非地理上广泛分布且高度保守。
Theileria parvais a tick‐transmitted apicomplexan protozoan parasite that infects lymphocytes of cattle and African Cape buffalo (Syncerus caffer), causing a frequently fatal disease of cattle in eastern, central and southern Africa. A live vaccination procedure, known as infection and treatment method (ITM), the most frequently used version of which comprises the Muguga, Serengeti‐transformed and Kiambu 5 stocks ofT. parva, delivered as a trivalent cocktail, is generally effective. However, it does not always induce 100% protection against heterologous parasite challenge. Knowledge of the genetic diversity ofT. parvain target cattle populations is therefore important prior to extensive vaccine deployment. This study investigated the extent of genetic diversity withinT. parvafield isolates derived from Ankole (Bos taurus) cattle in south‐western Uganda using 14 variable number tandem repeat (VNTR) satellite loci and the sequences of two antigen‐encoding genes that are targets of CD8+T‐cell responses induced by ITM, designated Tp1 and Tp2. The findings revealed aT. parvaprevalence of 51% confirming endemicity of the parasite in south‐western Uganda. Cattle‐derivedT. parvaVNTR genotypes revealed a high degree of polymorphism. However, all of theT. parvaTp1 and Tp2 alleles identified in this study have been reported previously, indicating that they are widespread geographically in East Africa and highly conserved.
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影响因子：
2.6
作者：
Thumbi SM;Bronsvoort MB;Kiara H;Toye PG;Poole J;Ndila M;Conradie I;Jennings A;Handel IG;Coetzer JA;Steyl J;Hanotte O;Woolhouse ME
通讯作者：
Woolhouse ME
DOI：
--
发表时间：
2004
期刊：
影响因子：
--
作者：
J. Coetzer;R. Tustin
通讯作者：
R. Tustin
乌干达西部不同畜牧系统下牛中泰勒虫和巴贝虫的流行率和特征。
DOI：
--
发表时间：
2010
期刊：
影响因子：
--
作者：
D. Muhanguzi;E. Matovu;C. Waiswa
通讯作者：
C. Waiswa
编码 CD8 T 细胞识别的两种细小泰勒虫抗原的基因在南苏丹牛群中表现出序列多样性，但大多数等位基因与活疫苗混合物的 Muguga 成分相似
影响因子：
3.7
作者：
D. Salih;R. Pelle;J. Mwacharo;M. Njahira;W. L. Marcellino;H. Kiara;Agol K Malak;A. E. El Hussein;R. Bishop;R. Skilton
通讯作者：
R. Skilton
影响因子：
3.2
作者：
Obara, Isaiah;Nielsen, Morten;Bishop, Richard P.
通讯作者：
Bishop, Richard P.