Hyperspectral computed tomographic imaging spectroscopy of vascular oxygen gradients in the rabbit retina in vivo.
Hyperspectral computed tomographic imaging spectroscopy of vascular oxygen gradients in the rabbit retina in vivo.
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DOI：
10.1371/journal.pone.0024482
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Humayun MS
中科院分区：
文献类型：
--
作者：
Kashani AH;Kirkman E;Martin G;Humayun MS
视网膜血管疾病的诊断取决于检眼镜检查结果，这些结果通常发生在严重视力丧失（如静脉阻塞）或不可逆的慢性变化（如糖尿病视网膜病变）之后。尽管最近取得了进展，但诊断成像目前对血管功能和局部氧气输送的揭示很少。血管功能的一种潜在有用的测量是血红蛋白氧含量的测量。在本文中，我们展示了一种新的方法，准确，快速，方便地测量视网膜血管内的氧饱和度使用在体内成像光谱。该方法使用耦合到二维衍射光学器件的市售眼底相机，该二维衍射光学器件将入射光以校准图案散射到焦平面阵列上。使用计算机断层摄影算法将衍射光谱图案重建成原始图像的波长分量。在本文中，氧合和脱氧血红蛋白的光谱成分进行了分析，从图像内的血管。仅在几毫秒内就可以进行多达76次光谱测量，并用于量化10-15度视野内视网膜血管内的氧饱和度。这里描述的方法可以在比常规血氧测定系统少得多的时间内采集10倍的光谱数据（同时利用普遍接受的眼底相机平台）。将该方法应用于视网膜血管疾病的动物模型和临床受试者，将为视网膜血管疾病和生理学提供有用和新颖的信息。
Diagnosis of retinal vascular diseases depends on ophthalmoscopic findings that most often occur after severe visual loss (as in vein occlusions) or chronic changes that are irreversible (as in diabetic retinopathy). Despite recent advances, diagnostic imaging currently reveals very little about the vascular function and local oxygen delivery. One potentially useful measure of vascular function is measurement of hemoglobin oxygen content. In this paper, we demonstrate a novel method of accurately, rapidly and easily measuring oxygen saturation within retinal vessels using in vivo imaging spectroscopy. This method uses a commercially available fundus camera coupled to two-dimensional diffracting optics that scatter the incident light onto a focal plane array in a calibrated pattern. Computed tomographic algorithms are used to reconstruct the diffracted spectral patterns into wavelength components of the original image. In this paper the spectral components of oxy- and deoxyhemoglobin are analyzed from the vessels within the image. Up to 76 spectral measurements can be made in only a few milliseconds and used to quantify the oxygen saturation within the retinal vessels over a 10–15 degree field. The method described here can acquire 10-fold more spectral data in much less time than conventional oximetry systems (while utilizing the commonly accepted fundus camera platform). Application of this method to animal models of retinal vascular disease and clinical subjects will provide useful and novel information about retinal vascular disease and physiology.
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影响因子：
3.5
作者：
Hammer, Martin;Vilser, Walthard;Schweitzer, Dietrich
通讯作者：
Schweitzer, Dietrich
影响因子：
1.2
作者：
Hirohara, Yoko;Okawa, Yoshitaka;Fujikado, Takashi
通讯作者：
Fujikado, Takashi
影响因子：
6.9
作者：
Blackburn, George Alan
通讯作者：
Blackburn, George Alan
DOI：
10.1007/s00417-009-1078-6
发表时间：
2009-08-01
影响因子：
2.7
作者：
Hammer, Martin;Vilser, Walthard;Strobel, Juergen
通讯作者：
Strobel, Juergen
影响因子：
1.9
作者：
DESCOUR, M;DERENIAK, E
通讯作者：
DERENIAK, E