Involvement of the skin during bluetongue virus infection and replication in the ruminant host.
Involvement of the skin during bluetongue virus infection and replication in the ruminant host.
复制标题
DOI：
10.1186/1297-9716-43-40
复制
发表时间：
2012-04-30
影响因子：
4.4
通讯作者：
Mertens PP
中科院分区：
文献类型：
--
作者：
Darpel KE;Monaghan P;Simpson J;Anthony SJ;Veronesi E;Brooks HW;Elliott H;Brownlie J;Takamatsu HH;Mellor PS;Mertens PP
蓝舌病毒（BTV）是一种双链（ds）RNA病毒（环状病毒属;呼肠孤病毒科），其被认为能够感染所有种类的家养和野生反刍动物，尽管临床症状主要见于绵羊。BTV是节肢动物传播的（“虫媒病毒”），并且能够在昆虫和哺乳动物宿主的许多不同细胞类型中进行生产性感染和复制。尽管BTV在反刍动物中的器官和细胞嗜性已经成为几项研究的主题，但其发病机制的许多方面仍然知之甚少，部分原因是在区分“病毒复制”和“病毒存在”方面存在固有的问题。在广泛的感染绵羊组织中研究了BTV的复制和器官嗜性，通过使用共聚焦显微镜对非结构蛋白或结构蛋白（分别为NS 2或VP 7和核心蛋白）进行免疫荧光标记，以区分病毒的存在和复制。这些结果进行了比较，在选定的器官从感染的羊的肉眼和显微镜下的病理学发现。在两种主要细胞类型中证实了复制：血管内皮细胞和形态上类似于淋巴细胞、单核细胞/巨噬细胞和/或树突状细胞的无颗粒白细胞。两个器官（皮肤和扁桃体）被证明支持相对较高水平的BTV复制，尽管它们以前没有被提出作为BTV感染期间的重要复制位点。BTV在皮肤中的高水平复制被认为是BTV的发病机制和传播（通过叮咬昆虫）的重要意义，并讨论了我们目前的BTV发病机制模型的改进。
Bluetongue virus (BTV) is a double stranded (ds) RNA virus (genus Orbivirus; family Reoviridae), which is considered capable of infecting all species of domestic and wild ruminants, although clinical signs are seen mostly in sheep. BTV is arthropod-borne (“arbovirus”) and able to productively infect and replicate in many different cell types of both insects and mammalian hosts. Although the organ and cellular tropism of BTV in ruminants has been the subject of several studies, many aspects of its pathogenesis are still poorly understood, partly because of inherent problems in distinguishing between “virus replication” and “virus presence”.BTV replication and organ tropism were studied in a wide range of infected sheep tissues, by immuno-fluorescence-labeling of non-structural or structural proteins (NS2 or VP7 and core proteins, respectively) using confocal microscopy to distinguish between virus presence and replication. These results are compared to gross and microscopic pathological findings in selected organs from infected sheep. Replication was demonstrated in two major cell types: vascular endothelial cells, and agranular leukocytes which morphologically resemble lymphocytes, monocytes/macrophages and/or dendritic cells. Two organs (the skin and tonsils) were shown to support relatively high levels of BTV replication, although they have not previously been proposed as important replication sites during BTV infection. The high level of BTV replication in the skin is thought to be of major significance for the pathogenesis and transmission of BTV (via biting insects) and a refinement of our current model of BTV pathogenesis is discussed.
登录
查看更多内容
影响因子：
2.4
作者：
MacLachlan, N. J.;Crafford, J. E.;Venter, E. H.
通讯作者：
Venter, E. H.
影响因子：
3.8
作者：
DeMaula, CD;Jutila, MA;MacLachlan, NJ
通讯作者：
MacLachlan, NJ
影响因子：
3.8
作者：
BARRATTBOYES, SM;ROSSITTO, PV;MACLACHLAN, NJ
通讯作者：
MACLACHLAN, NJ
影响因子：
5.4
作者：
Brodie, SJ;Bardsley, KD;Wilson, WC
通讯作者：
Wilson, WC
德国蓝舌病毒血清型 8 的流行病学。
影响因子：
11.8
作者：
Conraths FJ;Gethmann JM;Staubach C;Mettenleiter TC;Beer M;Hoffmann B
通讯作者：
Hoffmann B