Chromosomal analysis of non-small-cell lung cancer by multicolour fluorescent in situ hybridisation.
Chromosomal analysis of non-small-cell lung cancer by multicolour fluorescent in situ hybridisation.
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DOI：
10.1038/sj.bjc.6601569
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发表时间：
2004-02-23
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
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作者：
非小细胞肺癌的细胞遗传学异常仍然难以捉摸，主要是由于难以获得实体瘤的中期扩散。我们已经使用多色荧光原位杂交（M-FISH）和比较基因组杂交（CGH）的分子细胞遗传学技术，分析四个原发性非小细胞肺癌样本和两个已建立的细胞系（COR-L23和COR-L105），以确定常见的染色体畸变。CGH显示5 p、3q、8 q、11 q、2 q、12 p和12 q上的区域通常被过度代表，而9 p、3 p、6 q、17 p、22 q、8 p、10 p、10 q和19 p上的区域通常被代表不足。M-FISH显示了许多复杂的染色体重排。在6个样品中的3个中观察到5号和14号、5号和11号以及1号和6号染色体之间的易位，在每个样品中观察到另外14个易位。Y染色体的丢失和20号和5 p染色体的获得也很常见。4、5、8、11、12和19号染色体最常发生染色体间易位。对复发性畸变的进一步研究将是必要的，以确定所涉及的特定断点以及它们在非小细胞肺癌的病因学、诊断和预后中可能发挥的任何作用。
The cytogenetic abnormalities in non-small-cell lung cancer remain elusive due primarily to the difficulty in obtaining metaphase spreads from solid tumours. We have used the molecular cytogenetic techniques of multicolour fluorescent in situ hybridisation (M-FISH) and comparative genomic hybridisation (CGH) to analyse four primary non-small-cell lung cancer samples and two established cell lines (COR-L23 and COR-L105) in order to identify common chromosomal aberrations. CGH revealed regions on 5p, 3q, 8q, 11q, 2q, 12p and 12q to be commonly over-represented and regions on 9p, 3p, 6q, 17p, 22q, 8p, 10p, 10q and 19p to be commonly under-represented. M-FISH revealed numerous complex chromosomal rearrangements. Translocations between chromosomes 5 and 14, 5 and 11 and 1 and 6 were observed in three of the six samples, with a further 14 translocations being observed in two samples each. Loss of the Y chromosome and gains of chromosomes 20 and 5p were also frequent. Chromosomes 4, 5, 8, 11, 12 and 19 were most frequently involved in interchromosomal translocations. Further investigation of the recurrent aberrations will be necessary to identify the specific breakpoints involved and any role they may have in the aetiology, diagnosis and prognosis of non-small-cell lung cancer.
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影响因子：
56.9
作者：
Schrock, E;duManoir, S;Ried, T
通讯作者：
Ried, T
影响因子：
30.8
作者：
Speicher, MR;Ballard, SG;Ward, DC
通讯作者：
Ward, DC
影响因子：
6.4
作者：
Ashman, JNE;Brigham, J;Cawkwell, L
通讯作者：
Cawkwell, L
影响因子：
7.3
作者：
Goeze, A;Schlüns, K;Petersen, I
通讯作者：
Petersen, I
影响因子：
--
作者：
Luk, C;Tsao, MS;Squire, JA
通讯作者：
Squire, JA