Serum MicroRNA Profiles Serve as Novel Biomarkers for the Diagnosis of Alzheimer's Disease.
Serum MicroRNA Profiles Serve as Novel Biomarkers for the Diagnosis of Alzheimer's Disease.
复制标题
血清 MicroRNA 谱可作为诊断阿尔茨海默病的新型生物标志物。
DOI：
10.1155/2015/625659
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Zhang CY
中科院分区：
文献类型：
--
作者：
Dong H;Li J;Huang L;Chen X;Li D;Wang T;Hu C;Xu J;Zhang C;Zen K;Xiao S;Yan Q;Wang C;Zhang CY
阿尔茨海默病（AD）是最常见的痴呆类型，及时诊断AD对于延缓疾病发展和提高患者生活质量至关重要。然而，AD检测，特别是在早期阶段，由于缺乏特异性生物标志物，仍然是一个巨大的挑战。本研究旨在鉴定和验证循环miRNA作为AD新生物标志物的潜力。采用Solexa测序法筛选AD患者和对照组血清中miRNAs的表达谱。RT-qPCR用于在个体水平上确认改变的miRNA。此外，在轻度认知障碍（MCI）和血管性痴呆（VD）患者的血清样品中检测候选miRNAs。结果显示，与对照组相比，AD患者血清中的4种miRNA（miR-31、miR-93、miR-143和miR-146 a）显著降低。受试者工作特征曲线分析表明，这四种miRNAs可用作AD的潜在生物标志物。此外，与对照组相比，MCI中的miR-93和miR-146 a显著升高，并且miR-31、miR-93和miR-146 a的组可用于区分AD和VD。我们建立了一组四种血清miRNAs作为诊断AD的新的非侵入性生物标志物。
Alzheimer's disease (AD) is the most common type of dementia, and promptly diagnosis of AD is crucial for delaying the development of disease and improving patient quality of life. However, AD detection, particularly in the early stages, remains a substantial challenge due to the lack of specific biomarkers. The present study was undertaken to identify and validate the potential of circulating miRNAs as novel biomarkers for AD. Solexa sequencing was employed to screen the expression profile of serum miRNAs in AD and controls. RT-qPCR was used to confirm the altered miRNAs at the individual level. Moreover, candidate miRNAs were examined in the serum samples of patients with mild cognitive impairment (MCI) and vascular dementia (VD). The results showed that four miRNAs (miR-31, miR-93, miR-143, and miR-146a) were markedly decreased in AD patients' serum compared with controls. Receiver operating characteristic curve analysis demonstrated that this panel of four miRNAs could be used as potential biomarker for AD. Furthermore, miR-93, and miR-146a were significantly elevated in MCI compared with controls, and the panel of miR-31, miR-93 and miR-146a can be used to discriminate AD from VD. We established a panel of four serum miRNAs as a novel noninvasive biomarker for AD diagnosis.
登录
查看更多内容
影响因子：
4.7
作者：
Resnick, Kimberly E.;Alder, Hansjuerg;Cohn, David E.
通讯作者：
Cohn, David E.
影响因子：
14
作者：
Brookmeyer, Ron;Johnson, Elizabeth;Arrighi, H. Michael
通讯作者：
Arrighi, H. Michael
DOI：
10.1073/pnas.0710263105
发表时间：
2008-04-29
影响因子：
11.1
作者：
Hebert, Sebastien S.;Horre, Katrien;De Strooper, Bart
通讯作者：
De Strooper, Bart
影响因子：
3.7
作者：
Nunez-Iglesias J;Liu CC;Morgan TE;Finch CE;Zhou XJ
通讯作者：
Zhou XJ
血清 microRNA 是有前途的弥漫性大 B 细胞淋巴瘤的新型生物标志物
影响因子：
3.5
作者：
Fang, Cheng;Zhu, Dan-Xia;Li, Jian-Yong
通讯作者：
Li, Jian-Yong