Random mutagenesis analysis and identification of a novel C(2)H(2)-type transcription factor from the nematode-trapping fungus Arthrobotrys oligospora.
Random mutagenesis analysis and identification of a novel C(2)H(2)-type transcription factor from the nematode-trapping fungus Arthrobotrys oligospora.
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线虫捕获真菌少孢节孢菌中新型 C(2)H(2-) 型转录因子的随机诱变分析和鉴定
DOI：
10.1038/s41598-017-06075-5
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发表时间：
2017-07-17
影响因子：
4.6
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Jiang D;Zhou J;Bai G;Xing X;Tang L;Yang X;Li J;Zhang KQ;Yang J
少孢节孢菌是一种典型的捕食线虫真菌。本研究中，A.通过REMI（限制性内切酶介导的整合）方法获得寡孢酵母，并分析9个转化体的表型特性。9个转化体在生长、分生孢子形成、陷阱形成、胁迫耐受性和/或致病性方面彼此之间以及与亲本野生型菌株（WT）的差异。在转化体X5和X13中鉴定了hph盒的插入位点。在X5中，将盒插入AOL_s00076g273（76g273）和AOL_s00076g274（76g274）之间的非编码区中，并且在X5中76g274而非76g273的转录增强。76 g274 p具有两个保守结构域，并被预测为核蛋白，我们通过使用绿色荧光蛋白融合的76 g274 p在酿酒酵母中的核定位证实了这一点。76g274的转录受多种环境因子的刺激或抑制。76g274缺陷型突变体的产孢量降低了70%，并且与WT相比，产孢相关基因的转录在产孢过程中严重减少。综上所述，建立了一种筛选突变体的方法。利用该方法，我们鉴定了一个新的C2H2型转录因子，该因子正调控A.寡孢菌。
Arthrobotrys oligospora is a typical nematode-trapping fungus. In this study, 37 transformants of A. oligospora were obtained by REMI (restriction enzyme mediated integration) method and phenotypic properties of nine transformants were analyzed. The nine transformants showed differences in growth, conidiation, trap formation, stress tolerance, and/or pathogenicity among each other and with those of the parental wild-type strain (WT). The insertional sites of the hph cassette were identified in transformants X5 and X13. In X5, the cassette was inserted in the non-coding region between AOL_s00076g273 (76g273) and AOL_s00076g274 (76g274) and the transcription of 76g274, but not 76g273, was enhanced in X5. 76g274p had two conserved domains and was predicted as a nucleoprotein, which we confirmed by its nuclear localization in Saccharomyces cerevisiae using the green fluorescent protein-fused 76g274p. The transcription of 76g274 was stimulated or inhibited by several environmental factors. The sporulation yields of 76g274-deficient mutants were decreased by 70%, and transcription of several sporulation-related genes was severely diminished compared to the WT during the conidiation. In summary, a method for screening mutants was established in A. oligospora and using the method, we identified a novel C2H2-type transcription factor that positively regulates the conidiation of A. oligospora.
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影响因子：
16.5
作者：
Krijgsheld P;Bleichrodt R;van Veluw GJ;Wang F;Müller WH;Dijksterhuis J;Wösten HA
通讯作者：
Wösten HA
影响因子：
14.9
作者：
Mitchell A;Chang HY;Daugherty L;Fraser M;Hunter S;Lopez R;McAnulla C;McMenamin C;Nuka G;Pesseat S;Sangrador-Vegas A;Scheremetjew M;Rato C;Yong SY;Bateman A;Punta M;Attwood TK;Sigrist CJ;Redaschi N;Rivoire C;Xenarios I;Kahn D;Guyot D;Bork P;Letunic I;Gough J;Oates M;Haft D;Huang H;Natale DA;Wu CH;Orengo C;Sillitoe I;Mi H;Thomas PD;Finn RD
通讯作者：
Finn RD
影响因子：
2.5
作者：
Hagiwara, Daisuke;Kondo, Atsushi;Abe, Keietsu
通讯作者：
Abe, Keietsu
影响因子：
3.6
作者：
Kwon, Nak-Jung;Garzia, Aitor;Yu, Jae-Hyuk
通讯作者：
Yu, Jae-Hyuk
影响因子：
14.9
作者：
Franceschini A;Szklarczyk D;Frankild S;Kuhn M;Simonovic M;Roth A;Lin J;Minguez P;Bork P;von Mering C;Jensen LJ
通讯作者：
Jensen LJ