Ozone-induced dissociation on a traveling wave high-resolution mass spectrometer for determination of double-bond position in lipids.
Ozone-induced dissociation on a traveling wave high-resolution mass spectrometer for determination of double-bond position in lipids.
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DOI：
10.1002/rcm.7920
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发表时间：
2017-09-15
期刊：
影响因子：
--
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Vu N;Brown J;Giles K;Zhang Q
C=C在脂肪酰基链中的位置影响脂质的生物学功能。臭氧诱导解离-质谱（OzID-MS）在测定脂质双键位置方面具有很大的潜力，但通常在低分辨率离子阱质谱仪上实现。此外，迄今为止进行的大多数OzID-MS实验都集中在脂质的钠化加合物上，对基于LC-MS的脂质组学工作流程中产生的最常见质子化离子的碎片化研究较少。将从臭氧发生器在线产生的臭氧连接到Synapt G2高分辨率质谱仪的捕集器和转移气体供应管线。采用电喷雾电离直接输注法制备不同磷脂酰胆碱（PC）的质子化离子。不同的参数，包括行波的高度和速度，陷阱入口和直流电位进行了调整，以最大限度地提高OzID效率。比较了脂质的Sn-位置异构体和顺/反异构体与臭氧的反应性。行波的高度和速度进行调整，以延长脂质离子和臭氧之间的相遇时间，并导致改善OzID效率，因为没有增加捕获区DC和入口电位。在优化设置下，与单不饱和PC标准品的默认设置相比，OzID效率至少提高了1000倍。在sn-2 PC异构体中的单不饱和C=C比sn-1异构体更快地与臭氧反应。同样，反式PC中的C=C比顺式PC反应更快。这是OzID在行波使能高分辨率质谱仪的捕获和转移区域中的第一个实现。OzID的反应效率显着提高，通过减慢离子在陷阱区与臭氧的长期相互作用。这将有助于高分辨率OzID-MS在脂质组学中的应用。
The position of C=C within fatty acyl chains affects the biological function of lipids. Ozone induced dissociation-mass spectrometry (OzID-MS) has great potential in determination of lipid double bond position, but has generally been implemented on low resolution ion trap mass spectrometers. In addition, most of the OzID-MS experiments carried out so far were focused on the sodiated adducts of lipids, fragmentation of the most commonly observed protonated ions generated in LC-MS based lipidomics workflow has been less explored. Ozone generated in line from an ozone generator was connected to the trap and transfer gas supply line of a Synapt G2 high resolution mass spectrometer. Protonated ions of different phosphatidylcholines (PC) were generated by electrospray ionization through direct infusion. Different parameters, including traveling wave height and velocity, trap entrance and DC potential were adjusted to maximize the OzID efficiency. sn- positional isomers and cis/trans isomers of lipids were compared for their reactivity with ozone. Traveling Wave height and velocity were tuned to prolong the encounter time between lipid ions and ozone, and resulted in improved OzID efficiency, as did increasing trapping region DC and entrance potential. Under optimized settings, at least 1000 times enhancement in OzID efficiency was achieved compared to that under default settings for monounsaturated PC standards. Monounsaturated C=C in the sn-2 PC isomer reacted faster with ozone than the sn-1 isomer. Similarly, the C=C in trans PC reacted faster than in cis PC. This is the first implementation of OzID in the trap and transfer region of a traveling wave enabled high resolution mass spectrometer. The OzID reaction efficiency is significantly improved by slowing down ions in the trap region for their prolonged interaction with ozone. This will facilitate application of high resolution OzID-MS in lipidomics.
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影响因子：
2.3
作者：
Hsu, Fong-Fu;Kuhlmann, F. Matthew;Beverley, Stephen M.
通讯作者：
Beverley, Stephen M.
影响因子：
4.2
作者：
Pham, Huong T.;Maccarone, Alan T.;Blanksby, Stephen J.
通讯作者：
Blanksby, Stephen J.
影响因子：
2
作者：
Giles, Kevin;Williams, Jonathan P.;Campuzano, Iain
通讯作者：
Campuzano, Iain
DOI：
10.1016/s1044-0305(99)00035-5
发表时间：
1999-07-01
影响因子：
3.2
作者：
Hsu, FF;Turk, J
通讯作者：
Turk, J
影响因子：
14.8
作者：
Brown, H. Alex;Murphy, Robert C.
通讯作者：
Murphy, Robert C.