Strategies for Wheat Stripe Rust Pathogenicity Identified by Transcriptome Sequencing.
Strategies for Wheat Stripe Rust Pathogenicity Identified by Transcriptome Sequencing.
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DOI：
10.1371/journal.pone.0067150
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Rathjen JP
中科院分区：
文献类型：
--
作者：
Garnica DP;Upadhyaya NM;Dodds PN;Rathjen JP
由真菌条锈菌 f.sp. 引起的条锈病。小麦 (Pst) 是全球小麦生产的主要限制因素。 Pst 致病性背后的分子事件很大程度上是未知的。与所有锈病一样，Pst 在宿主细胞内创建一种称为吸器的特殊细胞结构，以从小麦中获取营养，并分泌称为效应蛋白的致病因子。我们纯化了 Pst 吸器，并使用下一代测序平台来组装吸器转录组以及萌发孢子的转录组。从 454 焦磷酸测序数据组装了 12,282 个转录本，并用作数字基因表达分析的参考，以基于 Illumina RNAseq 数据比较萌发的热孢子和吸器转录组。从吸器转录组中鉴定出超过 400 个编码构成候选效应子的分泌蛋白的基因，与萌发的孢子相比，其中三分之二在该组织中表达上调。 RT-PCR 分析证实了 94 个候选效应子的表达模式。分析还表明，孢子主要依靠储存的能量储备来生长和发育，而吸器则吸收宿主的营养物质，为生物合成途径和孢子的最终生产提供大量能量。总之，这些研究大大增加了我们对潜在 Pst 效应子的了解，并为这种重要生物体的致病策略提供了新的见解。
Stripe rust caused by the fungus Puccinia striiformis f.sp. tritici (Pst) is a major constraint to wheat production worldwide. The molecular events that underlie Pst pathogenicity are largely unknown. Like all rusts, Pst creates a specialized cellular structure within host cells called the haustorium to obtain nutrients from wheat, and to secrete pathogenicity factors called effector proteins. We purified Pst haustoria and used next-generation sequencing platforms to assemble the haustorial transcriptome as well as the transcriptome of germinated spores. 12,282 transcripts were assembled from 454-pyrosequencing data and used as reference for digital gene expression analysis to compare the germinated uredinospores and haustoria transcriptomes based on Illumina RNAseq data. More than 400 genes encoding secreted proteins which constitute candidate effectors were identified from the haustorial transcriptome, with two thirds of these up-regulated in this tissue compared to germinated spores. RT-PCR analysis confirmed the expression patterns of 94 effector candidates. The analysis also revealed that spores rely mainly on stored energy reserves for growth and development, while haustoria take up host nutrients for massive energy production for biosynthetic pathways and the ultimate production of spores. Together, these studies substantially increase our knowledge of potential Pst effectors and provide new insights into the pathogenic strategies of this important organism.
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影响因子：
2.1
作者：
Broeker, K;Bernard, F;Moerschbacher, BM
通讯作者：
Moerschbacher, BM
影响因子：
9.8
作者：
Bozdech, Zbynek;Llinas, Manuel;Pulliam, Brian Lee;Wong, Edith D;Zhu, Jingchun;DeRisi, Joseph L
通讯作者：
DeRisi, Joseph L
DOI：
10.1007/978-1-61737-998-7_8
发表时间：
2011-01-01
期刊：
PLANT IMMUNITY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Catanzariti, Ann-Maree;Mago, Rohit;Dodds, Peter
通讯作者：
Dodds, Peter
影响因子：
2.8
作者：
Cid, VJ;Adamiková, L;Nombela, C
通讯作者：
Nombela, C
影响因子：
56.9
作者：
Dagdas, Yasin F.;Yoshino, Kae;Talbot, Nicholas J.
通讯作者：
Talbot, Nicholas J.