Constitutive apoptosis in equine peripheral blood neutrophils in vitro.
Constitutive apoptosis in equine peripheral blood neutrophils in vitro.
复制标题
DOI：
10.1016/j.tvjl.2014.08.029
复制
发表时间：
2014-12
期刊：
影响因子：
--
通讯作者：
McGorum BC
中科院分区：
文献类型：
--
作者：
Brazil TJ;Dixon PM;Haslett C;Murray J;McGorum BC
马外周血中性粒细胞在培养36 h内发生自发的时间依赖性组成性凋亡。经历凋亡的马中性粒细胞发展在许多细胞类型（包括人中性粒细胞）中观察到的凋亡的结构和功能特征。中性粒细胞凋亡减少酵母多糖激活血清刺激的化学发光，但保持响应佛波醇肉豆蔻酸乙酸酯。脂多糖、肿瘤坏死因子α和绵羊红细胞吞噬作用均能促进中性粒细胞凋亡，而地塞米松和C5 a来源的ZAS则能抑制中性粒细胞凋亡。马和人类中性粒细胞凋亡不同的反应，脂多糖和地塞米松的反应的时间依赖性。本研究的目的是观察马外周血中性粒细胞的组成性凋亡，包括评估通过改变组成性凋亡率可能调节中性粒细胞存活的因素。细胞经历自发的时间依赖性的组成性凋亡时，老化培养长达36小时，开发的结构和功能的特点，在许多细胞类型，包括人类中性粒细胞中观察到的凋亡。中性粒细胞凋亡也减少酵母多糖激活血清（ZAS）刺激的化学发光，但保持佛波醇肉豆蔻酸乙酸酯（PMA）的反应。脂多糖（LPS）、肿瘤坏死因子α（TNF α）和绵羊红细胞吞噬作用均能促进马中性粒细胞凋亡，而地塞米松（Dexamethasone，D甲酰-蛋氨酸-亮氨酸-苯丙氨酸、白三烯B4、血小板活化因子和PMA对马中性粒细胞凋亡无明显影响。马和人的中性粒细胞凋亡对LPS的反应和对地塞米松的反应的时间依赖性之间存在差异。
Equine peripheral blood neutrophils underwent spontaneous time-dependent constitutive apoptosis when aged in culture for up to 36 h. Equine neutrophils undergoing apoptosis develop the structural and functional features of apoptosis observed in many cell types, including human neutrophils. Neutrophils undergoing apoptosis had diminished zymosan activated serum-stimulated chemiluminescence, but maintained responsiveness to phorbol myristate acetate. The constitutive rate of neutrophil apoptosis was promoted by lipopolysaccharide, tumour necrosis factor α and phagocytosis of opsonised ovine erythrocytes, while it was inhibited by dexamethasone and ZAS (a source of C5a). Equine and human neutrophil apoptosis differed in terms of responses to lipopolysaccharide and in the time-dependence of the response to dexamethasone. The aim of this study was to characterise constitutive apoptosis in equine peripheral blood neutrophils, including assessment of factors that potentially modulate neutrophil survival through alteration of the rate of constitutive apoptosis. Cells underwent spontaneous time-dependent constitutive apoptosis when aged in culture for up to 36 h, developing the structural and functional features of apoptosis observed in many cell types, including human neutrophils. Neutrophils undergoing apoptosis also had diminished zymosan activated serum (ZAS)-stimulated chemiluminescence, but maintained responsiveness to phorbol myristate acetate (PMA). The constitutive rate of equine neutrophil apoptosis was promoted by lipopolysaccharide (LPS), tumour necrosis factor α and phagocytosis of opsonised ovine erythrocytes, while it was inhibited by dexamethasone and ZAS (a source of C5a). Formyl-Met-Leu-Phe, leukotriene B4, platelet activating factor and PMA had no demonstrable effect on equine neutrophil apoptosis. There was a difference between equine and human neutrophil apoptosis in response to LPS and the time-dependence of the response to dexamethasone.
登录
查看更多内容
影响因子：
6.1
作者：
Brazil, TJ;Dagleish, MP;Chilvers, ER
通讯作者：
Chilvers, ER
影响因子：
2.2
作者：
Kim, DY;Taylor, HW;Cho, DY
通讯作者：
Cho, DY
DOI：
10.1111/j.1939-1676.1991.tb00944.x
发表时间：
1991-05-01
影响因子：
2.6
作者：
MORRIS, DD
通讯作者：
MORRIS, DD
影响因子：
5.5
作者：
Brazil, TJ;Rossi, AG;Chilvers, ER
通讯作者：
Chilvers, ER
影响因子：
3.7
作者：
Hirsch G;Lavoie-Lamoureux A;Beauchamp G;Lavoie JP
通讯作者：
Lavoie JP