Monitoring angiogenesis noninvasively with near-infrared spectroscopy.

Monitoring angiogenesis noninvasively with near-infrared spectroscopy.
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使用近红外光谱无创监测血管生成。

DOI:
10.1117/1.3000431
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发表时间:
2008
影响因子:
3.5
通讯作者:
Shaw,RAnthony
Shaw,RAnthony
中科院分区:
医学3区
文献类型:
--
作者:
Dunn,JeffreyF;Zhang,Qiong;Wu,Ying;Srinivasan,Sathyanarayanan;Smith,MichaelR;Shaw,RAnthony

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近红外(NIR)光谱用于量化脑血容量(CBV),作为血管生成(新血管形成)的标志。将大鼠暴露于半个大气压的慢性低氧中刺激血管生成,用二阶微分近红外光谱分析血管生成前后脑组织总血红蛋白的含量。脑血红蛋白(来自宽带近红外光谱)以及大血管血红蛋白和红细胞压积(来自血液样本)被用于计算CBV的值。脑内总血红蛋白为(,)预习服,习服后增加72%。CBV最初为yv/v,驯化后增加31%。每只单独的动物都显示出可测量的CBV增加。本研究表明,近红外宽带光谱分析技术可用于CBV的重复测量,并可作为一种无创性方法应用于血管生成的研究。
Near-infrared (NIR) spectroscopy is used to quantify cerebral blood volume (CBV) as a marker of angiogenesis (formation of new blood vessels). Rats are exposed to chronic hypoxia forat half atmospheric pressure to stimulate angiogenesis, and second-differential NIR spectroscopy is used to quantify total cerebral hemoglobin before and after angiogenesis. The cerebral hemoglobin (from broadband NIR spectroscopy), and the large vessel hemoglobin and hematocrit (from blood samples), are used to derive values for the calculation of CBV. The total hemoglobin in brain is(,) preacclimation and increases by 72% postacclimation. CBV is initiallyv/v and increases by 31% with acclimation. Each individual animal shows a measureable increase in CBV. This study indicates that NIR broadband spectroscopy can be used for repeated measurements of CBV and can be applied as a noninvasive method to study angiogenesis.
DOI: --
发表时间: 1971
期刊: Journal of Physiology
影响因子: --
作者:
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DOI: 10.1083/jcb.40.2.305
发表时间: 1969-02
期刊: The Journal of cell biology
影响因子: --
作者:
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发表时间: 1967-01-01
期刊: BIOCHIMICA ET BIOPHYSICA ACTA
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作者:
ERNST, SA;GOERTEMI.CC;ELLIS, RA
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DOI: 10.1073/pnas.79.19.6033
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期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
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发表时间: 1978
影响因子: 3.6
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