Centrally administered adrenomedullin increases plasma oxytocin level with induction of c-fos messenger ribonucleic acid in the paraventricular and supraoptic nuclei of the rat.
Centrally administered adrenomedullin increases plasma oxytocin level with induction of c-fos messenger ribonucleic acid in the paraventricular and supraoptic nuclei of the rat.
复制标题
集中给药的肾上腺髓质素通过在大鼠的室旁核和视上核中诱导c-fos信使核糖核酸来增加血浆催产素水平。
作者：
R. Serino;Y. Ueta;Yuko Hara;M. Nomura;Yukiyo Yamamoto;Izumi Shibuya;Yukio Hattori;Kazuo Kitamura;K. Kangawa;John A. Russell;Hiroshi Yamashita
脑室内（i. c. v.）应用放免法、免疫组化法和原位杂交法观察了肾上腺髓质素（AM）对大鼠血浆催产素（OXT）、室旁核（PVN）和视上核（SON）内c-Fos蛋白（Fos）和c-Fos mRNA（mRNA）的影响。AM的中枢给药引起血浆OXT水平的显著增加。侧脑室注射AM可引起PVN和SON背侧部Fos样免疫反应性（LI）的明显诱导。在室旁核和视上核中，OXT样细胞主要表现为核Fos样阳性，而精氨酸加压素样阳性细胞较少。原位杂交组织化学结果显示，AM注射后30 min，PVN和SON中c-fos mRNA的表达呈剂量相关性增加。通过用AM受体拮抗剂人AM-（22-52）-NH 2预处理来降低这种诱导。这些结果表明，中央AM是负责通过选择性AM受体激活PVN和SON中的神经分泌细胞，并且AM通过激活下丘脑OXT产生细胞来刺激OXT的分泌。
The effects of intracerebroventricular (i.c.v.) administration of adrenomedullin (AM) on plasma oxytocin (OXT), c-Fos protein (Fos), and c-fos messenger RNA (mRNA) in the paraventricular (PVN) and supraoptic nuclei (SON) of the rat were investigated using RIA for OXT, immunohistochemistry for Fos, and in situ hybridization histochemistry for c-Fos mRNA. Central administration of AM caused a significant increase in the plasma OXT level. Intracerebroventricular administration of AM caused a marked induction of Fos-like immunoreactivity (LI) in the PVN and in the dorsal parts of the SON. In the PVN and SON, OXT-LI cells predominantly exhibited nuclear Fos-LI in comparison with arginine vasopressin-LI cells. In situ hybridization histochemistry revealed that the induction of c-fos mRNA in the PVN and SON was increased in a dose-related manner 30 min after i.c.v. administration of AM. This induction was reduced by pretreatment with the AM receptor antagonist, human AM-(22-52)-NH2. These results suggest that central AM is responsible for activating the neurosecretory cells in the PVN and SON via selective AM receptors, and that AM stimulates the secretion of OXT by activating hypothalamic OXT-producing cells.
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影响因子：
56.9
作者：
SAGAR, SM;SHARP, FR;CURRAN, T
通讯作者：
CURRAN, T
建立中枢催产素的行为和生理功能：催产素和摄入行为研究的见解。
影响因子：
--
作者：
Verbalis,JG;Blackburn,RE;Hoffman,GE;Stricker,EM
通讯作者：
Stricker,EM
影响因子：
56.9
作者：
VERBALIS, JG;MCCANN, MJ;STRICKER, EM
通讯作者：
STRICKER, EM
渗透压和容量刺激在大鼠神经垂体分泌调节中的相互作用。
DOI：
10.1152/ajpregu.1986.250.2.r267
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Stricker,EM;Verbalis,JG
通讯作者：
Verbalis,JG