Comparative study of methyl-CpG-binding domain proteins.
Comparative study of methyl-CpG-binding domain proteins.
复制标题
甲基-CPG结合结构域蛋白的比较研究。
DOI：
10.1186/1471-2164-4-1
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发表时间：
2003-01-16
期刊：
影响因子：
4.4
通讯作者：
Nuber, UA
中科院分区：
文献类型：
--
作者：
Roloff, TC;Ropers, HH;Nuber, UA
基因组DNA中CpG二核苷酸的甲基化是脊椎动物基因表达调控的基本表观遗传学机制。带有甲基CpG结合域(MBD)的蛋白质可以与单个甲基化的CPGS结合，其中大部分参与转录调控。到目前为止，已有5种脊椎动物MBD蛋白被描述为MBD家族成员：MBD1、MBD2、MBD3、MBD4和MECP2。我们对含有MBD的新蛋白质进行了数据库搜索，并鉴定了六个与前面描述的不同的氨基酸序列。在这里，我们提供了他们的MBD序列，额外的蛋白质基序和编码基因的表达的比较。计算的无根树形图表明，在这些蛋白质中至少存在四种不同的MBD组。其中两个多肽KIAA1461和KIAA1887仅作为基于人类部分cDNA的预测氨基酸序列存在。我们通过Northern杂交分析了它们的表达，发现它们在所有研究的8个正常组织中分别有~8kb和~5kb的转录本。在小鼠和人中均能鉴定出11个具有MBD的多肽。对蛋白质结构域的分析表明，它们中的大多数在转录调控中发挥作用。在Rett综合征的背景下，了解其他现有的MBD蛋白及其表达模式是重要的。
Methylation at CpG dinucleotides in genomic DNA is a fundamental epigenetic mechanism of gene expression control in vertebrates. Proteins with a methyl-CpG-binding domain (MBD) can bind to single methylated CpGs and most of them are involved in transcription control. So far, five vertebrate MBD proteins have been described as MBD family members: MBD1, MBD2, MBD3, MBD4 and MECP2. We performed database searches for new proteins containing an MBD and identified six amino acid sequences which are different from the previously described ones. Here we present a comparison of their MBD sequences, additional protein motifs and the expression of the encoding genes. A calculated unrooted dendrogram indicates the existence of at least four different groups of MBDs within these proteins. Two of these polypeptides, KIAA1461 and KIAA1887, were only present as predicted amino acid sequences based on a partial human cDNA. We investigated their expression by Northern blot analysis and found transcripts of ~8 kb and ~5 kb respectively, in all eight normal tissues studied. Eleven polypeptides with a MBD could be identified in mouse and man. The analysis of protein domains suggests a role in transcriptional regulation for most of them. The knowledge of additional existing MBD proteins and their expression pattern is important in the context of Rett syndrome.
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影响因子：
3.5
作者：
LaSalle, JM;Goldstine, J;Greco, CM
通讯作者：
Greco, CM
影响因子：
2.5
作者：
Hendrich, B;Abbott, C;Bird, A
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Bird, A
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11.4
作者：
Ohki, I;Shimotake, N;Shirakawa, M
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Shirakawa, M
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64.8
作者：
Nan, XS;Ng, HH;Bird, A
通讯作者：
Bird, A
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13.8
作者：
Doerks, T;Copley, R;Bork, P
通讯作者：
Bork, P