A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings.
A New and Sensitive HPLC-UV Method for Rapid and Simultaneous Quantification of Curcumin and D-Panthenol: Application to In Vitro Release Studies of Wound Dressings.
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DOI：
10.3390/molecules27061759
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发表时间：
2022-03-08
期刊：
影响因子：
--
通讯作者：
Larrañeta E
中科院分区：
文献类型：
--
作者：
Anjani QK;Utomo E;Domínguez-Robles J;Detamornrat U;Donnelly RF;Larrañeta E
姜黄素（CUR）和D-泛醇（DPA）已被广泛研究用于伤口愈合治疗。为了分析这两种化合物的剂型，如基于聚合物的伤口敷料或乳膏，一种分析方法，允许同时定量两种药物应开发。在这里，我们首次报告了一种经验证的高效液相色谱（HPLC）方法，结合UV检测，根据国际协调理事会（ICH）指南设定的标准定量CUR和DPA。使用C18色谱柱进行分析物分离，色谱柱使用由0.001% v/v磷酸和甲醇组成的移动的相，使用梯度法，运行时间为15 min。该方法在0.39-12.5 μg mL−1范围内对药物浓度呈线性（R2 = 0.9999），DPA为0.39-25 μg mL−1（R2 = 1）。经验证的方法具有精密度和准确度。此外，发现CUR和DPA溶液在特定储存条件下稳定。因此，我们建议，在这项研究中开发的HPLC-UV方法可能是非常有用的筛选配方的CUR和DPA在临床前设置通过体外释放研究。
Curcumin (CUR) and D-panthenol (DPA) have been widely investigated for wound-healing treatment. In order to analyse these two compounds from a dosage form, such as polymer-based wound dressings or creams, an analytical method that allows the quantification of both drugs simultaneously should be developed. Here, we report for the first time a validated high-performance liquid chromatographic (HPLC) method coupled with UV detection to quantify CUR and DPA based on the standards set by the International Council on Harmonization (ICH) guidelines. The separation of the analytes was performed using a C18 column that utilised a mobile phase consisting of 0.001% v/v phosphoric acid and methanol using a gradient method with a run time of 15 min. The method is linear for drug concentrations within the range of 0.39–12.5 μg mL−1 (R2 = 0.9999) for CUR and 0.39–25 μg mL−1 for DPA (R2 = 1). The validated method was found to be precise and accurate. Moreover, the CUR and DPA solution was found to be stable under specific storage conditions. We, therefore, suggest that the HPLC-UV method developed in this study may be very useful in screening formulations for CUR and DPA within a preclinical setting through in vitro release studies.
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影响因子：
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DOI：
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