Cardiovascular effects of pulmonary exposure to single-wall carbon nanotubes.
Cardiovascular effects of pulmonary exposure to single-wall carbon nanotubes.
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肺暴露于单壁碳纳米管的心血管效应。
DOI：
10.1289/ehp.9688
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发表时间：
2007-03
影响因子：
10.4
通讯作者：
Simeonova PP
中科院分区：
文献类型：
--
作者：
Li Z;Hulderman T;Salmen R;Chapman R;Leonard SS;Young SH;Shvedova A;Luster MI;Simeonova PP
工程纳米材料，如单壁碳纳米管（SWCNT），在不同的工业中正在成为重要的技术。SWCNTs独特的物理特性和肺毒性引起了人们的关注，即呼吸道暴露于这些材料可能与心血管不良反应有关。在这些研究中，我们通过氧化应激试验评估了暴露于SWCNTs小鼠的主动脉线粒体改变，包括线粒体DNA的定量聚合酶链反应和斑块形成的形态计量学分析。单次咽内灌注SWCNTs诱导HO-1报告基因转基因小鼠肺、主动脉和心脏组织中血红素加氧酶-1 （HO-1）的激活，HO-1是氧化损伤的标志物。此外，我们发现暴露于SWCNT（10和40 μg/只）的C57BL/6小鼠在暴露后7、28和60天出现主动脉mtDNA损伤。线粒体dna损伤伴有主动脉线粒体谷胱甘肽和蛋白羰基水平的变化。由于这些修饰与心血管疾病相关，我们评估了重复暴露于SWCNTs （20 μg/小鼠，每隔一周1次，持续8周）是否会刺激ApoE−/−转基因小鼠动脉粥样硬化的进展。虽然swcnts暴露并没有改变这些小鼠的脂质谱，但它会导致喂食致动脉粥样硬化饮食的ApoE - / -小鼠加速斑块形成。在swcnts处理的小鼠中，用正面法测量的主动脉斑块面积和用组织病理学测量的头臂动脉斑块面积显著增加。这种反应伴随着mtDNA损伤的增加，但没有炎症。综上所述，这些发现具有足够的意义，值得进一步研究，以评估工作场所或环境暴露范式下swcnts的系统性影响。
Engineered nanosized materials, such as single-wall carbon nanotubes (SWCNT), are emerging as technologically important in different industries. The unique physical characteristics and the pulmonary toxicity of SWCNTs raised concerns that respiratory exposure to these materials may be associated with cardiovascular adverse effects. In these studies we evaluated aortic mitochondrial alterations by oxidative stress assays, including quantitative polymerase chain reaction of mitochondrial (mt) DNA and plaque formation by morphometric analysis in mice exposed to SWCNTs. A single intrapharyngeal instillation of SWCNTs induced activation of heme oxygenase-1 (HO-1), a marker of oxidative insults, in lung, aorta, and heart tissue in HO-1 reporter transgenic mice. Furthermore, we found that C57BL/6 mice, exposed to SWCNT (10 and 40 μg/mouse), developed aortic mtDNA damage at 7, 28, and 60 days after exposure. mtDNA damage was accompanied by changes in aortic mitochondrial glutathione and protein carbonyl levels. Because these modifications have been related to cardiovascular diseases, we evaluated whether repeated exposure to SWCNTs (20 μg/mouse once every other week for 8 weeks) stimulates the progression of atherosclerosis in ApoE−/− transgenic mice. Although SWCNT exposure did not modify the lipid profiles of these mice, it resulted in accelerated plaque formation in ApoE−/− mice fed an atherogenic diet. Plaque areas in the aortas, measured by the en face method, and in the brachiocephalic arteries, measured histopathologically, were significantly increased in the SWCNT-treated mice. This response was accompanied by increased mtDNA damage but not inflammation. Taken together, the findings are of sufficient significance to warrant further studies to evaluate the systemic effects of SWCNT under workplace or environmental exposure paradigms.
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影响因子：
37.8
作者：
Knight-Lozano, CA;Young, CG;Ballinger, SW
通讯作者：
Ballinger, SW
DOI：
10.1161/01.atv.14.4.605
发表时间：
1994-04-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
PALINSKI, W;ORD, VA;WITZTUM, JL
通讯作者：
WITZTUM, JL
DOI：
10.1152/ajplung.00078.2004
发表时间：
2004-10-01
影响因子：
4.9
作者：
Heckel, K;Kiefmann, R;Goetz, AE
通讯作者：
Goetz, AE
DOI：
10.1164/rccm.200508-1243pp
发表时间：
2006-03-01
影响因子：
24.7
作者：
O'Sullivan, BP;Michelson, AD
通讯作者：
Michelson, AD
影响因子：
10.4
作者：
Künzli N;Jerrett M;Mack WJ;Beckerman B;LaBree L;Gilliland F;Thomas D;Peters J;Hodis HN
通讯作者：
Hodis HN