Gibberellin biosynthesis and signal transduction is essential for internode elongation in deepwater rice.
Gibberellin biosynthesis and signal transduction is essential for internode elongation in deepwater rice.
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DOI：
10.1111/pce.12377
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Ashikari M
中科院分区：
文献类型：
--
作者：
Ayano M;Kani T;Kojima M;Sakakibara H;Kitaoka T;Kuroha T;Angeles-Shim RB;Kitano H;Nagai K;Ashikari M
在淹水条件下，深水水稻的叶片和节间可以延伸到水面以上，以捕获氧气，防止溺水。我们以前的研究表明，三个主要的数量性状基因座(QTL)控制着深水依赖的深水水稻节间伸长。在本研究中，我们研究了深水水稻节间伸长与年龄的关系。我们还利用一个QTL聚合系(NIL-1、 + 3、 + 12)，用生理和遗传方法研究了依赖深水的节间伸长与植物激素赤霉素(GA)的关系。在深水条件下，深水水稻在第6叶期之前没有节间伸长。此外，在第六叶期间，第六和第七节间发生了依赖深水的节间伸长。这些结果表明，深水水稻要到第六叶期才能开始节间伸长。超高效液相色谱串联质谱(UPLC-MS/MS)分析表明，GA1和GA4在深水水稻中的积累依赖于深水。此外，GA抑制剂还能抑制深水水稻的节间伸长。反之，在正常生长条件下，GA补饲模拟节间伸长。然而，GA生物合成和信号转导基因的突变阻止了依赖深水的节间伸长。这些数据表明，GA的生物合成和信号转导是深水依赖的深水水稻节间伸长所必需的。深水水稻获得了快速伸长节间以避免淹水和适应淹水条件的能力。它是如何调节节间伸长的？本文从生理和遗传两方面说明了植物激素赤霉素(GA)对节间伸长的调控作用。
Under flooded conditions, the leaves and internodes of deepwater rice can elongate above the water surface to capture oxygen and prevent drowning. Our previous studies showed that three major quantitative trait loci (QTL) regulate deepwater-dependent internode elongation in deepwater rice. In this study, we investigated the age-dependent internode elongation in deepwater rice. We also investigated the relationship between deepwater-dependent internode elongation and the phytohormone gibberellin (GA) by physiological and genetic approach using a QTL pyramiding line (NIL-1 + 3 + 12). Deepwater rice did not show internode elongation before the sixth leaf stage under deepwater condition. Additionally, deepwater-dependent internode elongation occurred on the sixth and seventh internodes during the sixth leaf stage. These results indicate that deepwater rice could not start internode elongation until the sixth leaf stage. Ultra-performance liquid chromatography tandem mass-spectrometry (UPLC-MS/MS) method for the phytohormone contents showed a deepwater-dependent GA1 and GA4 accumulation in deepwater rice. Additionally, a GA inhibitor abolished deepwater-dependent internode elongation in deepwater rice. On the contrary, GA feeding mimicked internode elongation under ordinary growth conditions. However, mutations in GA biosynthesis and signal transduction genes blocked deepwater-dependent internode elongation. These data suggested that GA biosynthesis and signal transduction are essential for deepwater-dependent internode elongation in deepwater rice. Deepwater rice obtained the ability for rapid internode elongation to avoid drowning and adapt to flooded condition. How does it regulate internode elongation? Using both physiological and genetic approach, this paper shows that the plant hormone, gibberellin (GA) regulates internode elongation.
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