Electrophysiological properties of basal forebrain cholinergic neurons identified by genetic and optogenetic tagging.
Electrophysiological properties of basal forebrain cholinergic neurons identified by genetic and optogenetic tagging.
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DOI：
10.1111/jnc.14073
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发表时间：
2017-08
影响因子：
4.7
通讯作者：
Role LW
中科院分区：
文献类型：
--
作者：
López-Hernández GY;Ananth M;Jiang L;Ballinger EC;Talmage DA;Role LW
神经元群体特异性的遗传修饰的小鼠系的产生的最新发展允许精确识别和选择性刺激体内胆碱能神经元。虽然大大减少费力比事后识别胆碱能神经元的免疫染色进行的研究，它是不知道是否遗传为基础的标记程序，允许在体内识别是电生理良性的。在这项研究中，我们使用携带BAC转基因的小鼠，该转基因驱动tau-GFP融合蛋白在胆碱能神经元中特异性表达。这使我们能够在急性切片制备中可视化基底前脑胆碱能神经元。使用全细胞，膜片钳电生理记录在急性脑片，在这里，我们提出了原始数据的基本电特性，这些基因标记的胆碱能神经元，包括放电率，静息膜电位，基强度和各种特性的动作电位和超极化后电位。基本的电特性进行了比较（1）与非胆碱能神经元在相同的大脑区域;（2）在胆碱能神经元之间的未成熟动物和年轻的成年人;和（3）与胆碱能神经元表达光敏通道。基于这些数据，我们的结论是：（a）胆碱能神经元的兴奋性低于其非胆碱能神经元，（B）胆碱能神经元的基本特性在青春期和青年期之间没有显著变化，（c）这些特性没有受到兴奋性视蛋白oChIEF慢性表达的显著影响。
Recent developments in the generation of neuronal population-specific, genetically-modified mouse lines have allowed precise identification and selective stimulation of cholinergic neurons in vivo. Although considerably less laborious than studies conducted with post-hoc identification of cholinergic neurons by immunostaining, it is not known whether the genetically based labeling procedures that permit in vivo identification are electrophysiologically benign. In this study we use mice carrying a BAC transgene that drives expression of a tau-GFP fusion protein specifically in cholinergic neurons. This allowed us to visualize basal forebrain cholinergic neurons in acute slice preparations. Using whole cell, patch clamp electrophysiological recording in acute brain slices, here we present original data about the basic electrical properties of these genetically tagged cholinergic neurons including firing rate, resting membrane potential, rheobase and various characteristics of their action potentials and after hyperpolarization potentials. The basic electrical properties are compared (1) with non-cholinergic neurons in the same brain regions; (2) in cholinergic neurons between immature animals and young adults; and (3) with cholinergic neurons that are expressing light sensitive channels. Our conclusions based on these data are (a) cholinergic neurons are less excitable then their non-cholinergic neighbors, (b) the basic properties of cholinergic neurons do not significantly change between adolescence and young adulthood and (c) these properties are not significantly affected by chronic expression of the excitatory opsin, oChIEF.
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影响因子：
3.4
作者：
Lin, John Y.;Lin, Michael Z.;Tsien, Roger Y.
通讯作者：
Tsien, Roger Y.
影响因子：
5.3
作者：
Unal, Cagri T.;Pare, Denis;Zaborszky, Laszlo
通讯作者：
Zaborszky, Laszlo
影响因子：
3.7
作者：
Hedrick T;Waters J
通讯作者：
Waters J
影响因子：
16.2
作者：
Jiang L;Kundu S;Lederman JD;López-Hernández GY;Ballinger EC;Wang S;Talmage DA;Role LW
通讯作者：
Role LW
影响因子：
3.3
作者：
Serafin, M;Williams, S;Muhlethaler, M
通讯作者：
Muhlethaler, M