Two distinct metacommunities characterize the gut microbiota in Crohn's disease patients.
Two distinct metacommunities characterize the gut microbiota in Crohn's disease patients.
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两个不同的元群落表征了克罗恩病患者的肠道微生物群
DOI：
10.1093/gigascience/gix050
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发表时间：
2017-07-01
期刊：
影响因子：
9.2
通讯作者：
Jia H
中科院分区：
文献类型：
--
作者：
He Q;Gao Y;Jie Z;Yu X;Laursen JM;Xiao L;Li Y;Li L;Zhang F;Feng Q;Li X;Yu J;Liu C;Lan P;Yan T;Liu X;Xu X;Yang H;Wang J;Madsen L;Brix S;Wang J;Kristiansen K;Jia H
炎症性肠道疾病克罗恩病(CD)已成为世界范围内的健康挑战。肠道微生物区系与宿主免疫系统密切相互作用，但其在CD中的功能影响尚不清楚。除了对少数CD患者的研究外，CD中肠道微生物区系的分析使用了16S rDNA扩增序列。在这里，我们使用元基因组鸟枪测序来提供CD微生物区系的组成和功能特征的详细特征，其中也包括未注释的细菌，并研究了排他性肠内营养对其调节。根据特征分类群，Cd微生物群落聚为两个不同的元群落，表明Cd微生物群落结构具有个体差异性。CD中元群落特有的功能变化表明，致炎的六酰化脂多糖变异体的产生者丰富，合成短链脂肪酸的可能性降低。Cd中生态网络的破坏很明显，同时许多细菌物种的生长速度也有所下降。短期排他性肠内营养对CD微生物区系的总体组成的影响有限，尽管在治疗后发生了功能变化。CD患者的微生物群可分为两个不同的元群落，其中受干扰最严重的元群落表现出明显偏离健康个体的功能潜力，这可能与CD的发病机制有关。
The inflammatory intestinal disorder Crohn's disease (CD) has become a health challenge worldwide. The gut microbiota closely interacts with the host immune system, but its functional impact in CD is unclear. Except for studies on a small number of CD patients, analyses of the gut microbiota in CD have used 16S rDNA amplicon sequencing. Here we employed metagenomic shotgun sequencing to provide a detailed characterization of the compositional and functional features of the CD microbiota, comprising also unannotated bacteria, and investigated its modulation by exclusive enteral nutrition. Based on signature taxa, CD microbiotas clustered into 2 distinct metacommunities, indicating individual variability in CD microbiome structure. Metacommunity-specific functional shifts in CD showed enrichment in producers of the pro-inflammatory hexa-acylated lipopolysaccharide variant and a reduction in the potential to synthesize short-chain fatty acids. Disruption of ecological networks was evident in CD, coupled with reduction in growth rates of many bacterial species. Short-term exclusive enteral nutrition elicited limited impact on the overall composition of the CD microbiota, although functional changes occurred following treatment. The microbiotas in CD patients can be stratified into 2 distinct metacommunities, with the most severely perturbed metacommunity exhibiting functional potentials that deviate markedly from that of the healthy individuals, with possible implication in relation to CD pathogenesis.
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DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
2.4
作者：
Ricanek, Petr;Lothe, Sheba M.;Tonjum, Tone
通讯作者：
Tonjum, Tone
影响因子：
2.7
作者：
Hermon-Taylor, J;Bull, TJ;Sumar, N
通讯作者：
Sumar, N
影响因子：
64.8
作者：
Park, Beom Seok;Song, Dong Hyun;Lee, Jie-Oh
通讯作者：
Lee, Jie-Oh
影响因子：
30.3
作者：
Gevers D;Kugathasan S;Denson LA;Vázquez-Baeza Y;Van Treuren W;Ren B;Schwager E;Knights D;Song SJ;Yassour M;Morgan XC;Kostic AD;Luo C;González A;McDonald D;Haberman Y;Walters T;Baker S;Rosh J;Stephens M;Heyman M;Markowitz J;Baldassano R;Griffiths A;Sylvester F;Mack D;Kim S;Crandall W;Hyams J;Huttenhower C;Knight R;Xavier RJ
通讯作者：
Xavier RJ