Using DNA-based stable isotope probing to reveal novel propionate- and acetate-oxidizing bacteria in propionate-fed mesophilic anaerobic chemostats
Using DNA-based stable isotope probing to reveal novel propionate- and acetate-oxidizing bacteria in propionate-fed mesophilic anaerobic chemostats
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使用基于 DNA 的稳定同位素探测揭示丙酸盐补给的中温厌氧恒化器中新型丙酸盐和乙酸盐氧化细菌
DOI：
10.1038/s41598-019-53849-0
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发表时间：
2019-11
影响因子：
4.6
通讯作者：
Yue-Qin Tang
中科院分区：
文献类型：
--
作者：
Hui-Zhong Wang;Xiao-Meng Lv;Yue Yi;Dan Zheng;Min Gou;Yong Nie;Bing Hu;Masaru K Nobu;Takashi Narihiro;Yue-Qin Tang
丙酸是厌氧发酵的重要中间产物之一。它的氧化进行的互养丙酸氧化细菌耦合氢营养产甲烷菌被认为是一个限速步骤甲烷生产。然而，由于纯培养分离的困难，目前对SPOB的了解是有限的。在本研究中，以丙酸盐作为唯一碳源的两个厌氧恒化器以不同的稀释率（0.05 d-1和0.15 d-1）运行。结合DNA稳定同位素探针（DNA-SIP）和16 SrRNA基因高通量测序技术，对两种产甲烷恒化器中的丙酸和乙酸氧化细菌进行了研究。13 C-丙酸盐/乙酸盐DNA-SIP的结果表明，Smithella，Syntrophobacterium，Cryptanaerobacterium，和未分类的Rhodocellaceae可能是推定的丙酸盐氧化细菌;未分类的Spirochaetaceae，未分类的Synergistaceae，未分类的Elusimicrobia，Mesotoga，和Gracilibacter可能有助于乙酸盐氧化;未分类的Syntrophaceae和Syntrophomonas可能是丁酸盐氧化菌。通过DNA-SIP，PL恒化器中16 S rRNA基因丰度高于总细菌的62%和PH恒化器中的38%的未分类OTU被揭示与丙酸盐降解相关。这些结果表明，各种未培养的细菌有助于厌氧消化过程中丙酸降解。这些细菌的功能和代谢特性需要进一步研究。
Propionate is one of the most important intermediates of anaerobic fermentation. Its oxidation performed by syntrophic propionate-oxidizing bacteria coupled with hydrogenotrophic methanogens is considered to be a rate-limiting step for methane production. However, the current understanding of SPOB is limited due to the difficulty of pure culture isolation. In the present study, two anaerobic chemostats fed with propionate as the sole carbon source were operated at different dilution rates (0.05 d−1 and 0.15 d−1). The propionate- and acetate-oxidizing bacteria in the two methanogenic chemostats were investigated combining DNA-stable isotope probing (DNA-SIP) and 16S rRNA gene high-throughput sequencing. The results of DNA-SIP with 13C-propionate/acetate suggested that, Smithella, Syntrophobacter, Cryptanaerobacter, and unclassified Rhodospirillaceae may be putative propionate-oxidizing bacteria; unclassified Spirochaetaceae, unclassified Synergistaceae, unclassified Elusimicrobia, Mesotoga, and Gracilibacter may contribute to acetate oxidation; unclassified Syntrophaceae and Syntrophomonas may be butyrate oxidizers. By DNA-SIP, unclassified OTUs with 16S rRNA gene abundance higher than 62% of total Bacteria in the PL chemostat and 38% in the PH chemostat were revealed to be related to the degradation of propionate. These results suggest that a variety of uncultured bacteria contribute to propionate degradation during anaerobic digestion. The functions and metabolic characteristics of these bacteria require further investigation.
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影响因子：
5.1
作者：
Lueders, T;Manefield, M;Friedrich, MW
通讯作者：
Friedrich, MW
影响因子：
3.4
作者：
Ahlert S;Zimmermann R;Ebling J;König H
通讯作者：
König H
DOI：
10.1038/ismej.2017.143
发表时间：
2018-01
期刊：
The ISME journal
影响因子：
--
作者：
Ziels RM;Sousa DZ;Stensel HD;Beck DAC
通讯作者：
Beck DAC
影响因子：
2.8
作者：
HOUWEN, FP;PLOKKER, J;ZEHNDER, AJB
通讯作者：
ZEHNDER, AJB
影响因子：
4.4
作者：
Gan, Yanlu;Qiu, Qiongfen;Lu, Yahai
通讯作者：
Lu, Yahai