Low CFB expression is independently associated with poor overall and disease-free survival in patients with lung adenocarcinoma.
Low CFB expression is independently associated with poor overall and disease-free survival in patients with lung adenocarcinoma.
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CFB 低表达与肺腺癌患者总体生存率和无病生存率较差独立相关
DOI：
10.3892/ol.2021.12739
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发表时间：
2021-06
期刊：
影响因子：
2.9
通讯作者：
Duan Y
中科院分区：
文献类型：
--
作者：
He C;Li Y;Zhang R;Chen J;Feng X;Duan Y
补体因子B （CFB）在补体系统的替代信号通路中起着关键作用，并在靶颗粒的标记中发挥关键作用，导致靶颗粒的有效清除。本研究旨在探讨CFB低表达水平与肺腺癌（LUAD）患者临床特征和生存状况的关系。患者数据基于rna测序和来自癌症基因组图谱数据库的临床数据。根据CFB的中位表达水平将所有患者分为两组。采用Kaplan-Meier曲线和单变量Cox回归分析探讨CFB与生存状态的关系。基因集富集分析检测CFB表达对信号通路损伤的影响。此外，利用逆转录定量PCR （RT-qPCR）和western blotting验证CFB在LUAD组织中的相对表达水平。数据显示，残余肿瘤分类、Karnofsky评分和肿瘤分期与总生存期相关，Karnofsky评分和分期与无病生存期相关。结果表明，根据连续和分类模型，CFB的高表达水平与患者总体生存率和无病生存率的增加有关。多变量分析结果表明，根据连续模型，CFB的高表达水平与总生存率和无病生存率的增加相关[风险比（HR）， 0.48；95%置信区间（95% CI）， 0.25-0.93；总生存率P=0.029；人力资源,0.29;95% ci, 0.15-0.59；在相应的协变量（残余肿瘤分类、Karnofsky表现评分和分期）校正后，分类模型（HR, 0.46; 95% CI, 0.22-0.93；总生存期P=0.031； HR, 0.25; 95% CI, 0.12-0.55；无病生存期P=0.001）。RT-qPCR和western blotting结果显示，与邻近非肿瘤组织相比，肺肿瘤组织中CFB mRNA和蛋白的相对表达水平下调。总的来说，目前的结果表明，CFB表达是LUAD患者总体生存和无病生存的独立预测因子。
Complement factor B (CFB) serves a pivotal role in the alternative signaling pathway of the complement system and exerts a key role in the labelling of target particles, resulting from effective clearance of the target. The present study aimed to investigate the association between low expression levels of CFB and the clinical features and survival status of patients with lung adenocarcinoma (LUAD). Patient data were based on RNA-sequencing and clinical data from The Cancer Genome Atlas database. All patients were divided into two groups based on the median expression of CFB. Kaplan-Meier curve and univariate Cox regression analyses were used to investigate the association between CFB and survival status. Gene set enrichment analysis was used to examine the effects of CFB expression on signaling pathway impairment. Furthermore, reverse transcription-quantitative PCR (RT-qPCR) and western blotting were used to verify the relative expression levels of CFB in LUAD tissues. The data revealed that residual tumor classification, Karnofsky performance score and cancer stage were associated with overall survival, and that Karnofsky performance score and stage were associated with disease-free survival. The results demonstrated that high expression levels of CFB were associated with increased patient overall and disease-free survival according to both continuous and categorical models. The results of multivariate analysis identified that high expression levels of CFB were associated with increased overall and disease-free survival according to both the continuous model [hazard ratio (HR), 0.48; 95% confidence interval (95% CI), 0.25–0.93; P=0.029 for overall survival; HR, 0.29; 95% CI, 0.15–0.59; P=0.001 for disease-free survival] and the categorical model (HR, 0.46; 95% CI, 0.22–0.93; P=0.031 for overall survival; HR, 0.25; 95% CI, 0.12–0.55; P=0.001 for disease-free survival) after adjusting for corresponding covariates (residual tumour classification, Karnofsky performance score and stage). Furthermore, the results of both RT-qPCR and western blotting indicated that the relative mRNA and protein expression levels of CFB in lung tumor tissues were downregulated compared with those in adjacent non-tumor tissues. Collectively, the present results suggested that CFB expression was an independent predictor of overall and disease-free survival in patients with LUAD.
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作者：
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