Effects of yak-activated protein on hematopoiesis and related cytokines in radiation-induced injury in mice.
Effects of yak-activated protein on hematopoiesis and related cytokines in radiation-induced injury in mice.
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牦牛激活蛋白对辐射损伤小鼠造血及相关细胞因子的影响
DOI：
10.3892/etm.2017.5256
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发表时间：
2017-12
影响因子：
2.7
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Duan Y;Yao X;Zhu J;Li Y;Zhang J;Zhou X;Qiao Y;Yang M;Li X
本研究旨在探讨牦牛激活蛋白对辐射损伤小鼠造血及细胞因子功能的保护作用。180只昆明种小鼠随机分为A、B、C三组。其中60只随机分为正常对照组、辐射模型组、阳性对照组和牦牛激活蛋白高、中、低剂量组（分别为10、5、2.5mg/kg）。其他120只小鼠用于辐射后第7天和第14天的后续实验。给药组小鼠口服Yak活化蛋白，正常对照组和辐射模型组小鼠口服等体积生理盐水，连续14 d。阳性对照组腹腔注射氨磷汀150 mg/kg。除对照组外，各组小鼠全身均匀接受5戈伊量的X射线照射一次。观察照射后外周血象、胸腺指数、脾脏指数、骨髓DNA含量、白细胞介素（IL）2和IL 6水平、B细胞淋巴瘤2（Bcl-2）和Bcl-2相关X蛋白（Bax）表达水平的变化。与对照组相比，牦牛激活蛋白低剂量组照射后14 d小鼠脾脏指数显著升高（P <0.05），牦牛激活蛋白高、中剂量组照射后14 d小鼠胸腺指数显著升高（P<0.05）。牦牛活化蛋白低剂量组血象指标逐渐升高，照射后7天明显高于模型组（P<0.05）。牦牛激活蛋白各剂量组小鼠骨髓DNA含量均明显升高，其中牦牛激活蛋白低剂量组照射后7 d较模型组显著升高（P<0.05）。牦牛激活蛋白组IL-2含量显著升高（P<0.05）。Bcl-2表达增强，Bax表达减弱（P<0.05）。这些结果表明牦牛激活蛋白对小鼠辐射损伤具有保护作用。牦牛激活蛋白在照射后14天的中剂量组效果最佳。
The aim of the present study was to investigate the protective effects of yak-activated protein on hematopoiesis and cytokine function in radiation-induced injury in mice. A total of 180 Kunming mice were randomly divided into three groups (A, B and C). Of these, 60 were randomly divided into a normal control group, a radiation model group, a positive control group and 3 yak-activated protein groups (high, medium and low dose groups; 10, 5 and 2.5 mg/kg, respectively). The other 120 mice were used for the subsequent experiments on days 7 and 14 following radiation. Yak-activated protein was administered orally to mice in the treatment groups and an equal volume of saline was administered orally to mice in the normal control and radiation model groups for 14 days. The positive control group received amifostine (150 mg/kg) via intraperitoneal injection. With the exception of the control group, the groups of mice received a 5 Gy quantity of X-radiation evenly over their whole body once. Changes in the peripheral hemogram, thymus and spleen indices, DNA content in the bone marrow, interleukin (IL)-2 and IL-6 levels, and the expression levels of B cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) following irradiation were assessed. The low dose of yak-activated protein significantly increased Spleen indices in mice 14 days after irradiation and the high and middle dose of yak-activated protein significantly increased Thymus indices in mice 14 days after irradiation (P<0.05) compared with the control group. In addition, hemogram results increased gradually in the low-yak-activated protein dose group and were significantly higher 7 days after irradiation compared with the radiation model group (P<0.05). The DNA content in the bone marrow was markedly increased in the yak-activated protein groups, and increased significantly in the low dose group at 7 days post-irradiation compared with the radiation model group (P<0.05). The IL-2 content was significantly increased in the yak-activated protein groups (P<0.05). Furthermore, Bcl-2 expression was increased and Bax expression was decreased (P<0.05). These results suggest that yak-activated protein exerts protective effects against radiation-induced injury in mice. The optimal effects of yak-activated protein were observed in the medium dose group 14 days after irradiation.
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影响因子：
8.8
作者：
Hospers, GAP;Eisenhauer, EA;de Vries, EGE
通讯作者：
de Vries, EGE
影响因子：
3.3
作者：
Schulte-Herbrüggen, O;Nassenstein, C;Braun, A
通讯作者：
Braun, A
DOI：
10.1016/j.mrgentox.2014.04.026
发表时间：
2014-08-01
影响因子：
1.9
作者：
Bhilwade, Hari N.;Jayakumar, S.;Chaubey, R. C.
通讯作者：
Chaubey, R. C.
影响因子：
3.3
作者：
Johnke, Roberta M.;Sattler, Jennifer A.;Allison, Ron R.
通讯作者：
Allison, Ron R.
影响因子：
5.4
作者：
Suh SS;Hwang J;Park M;Seo HH;Kim HS;Lee JH;Moh SH;Lee TK
通讯作者：
Lee TK