Anti-inflammation activities of mycosporine-like amino acids (MAAs) in response to UV radiation suggest potential anti-skin aging activity.
Anti-inflammation activities of mycosporine-like amino acids (MAAs) in response to UV radiation suggest potential anti-skin aging activity.
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DOI：
10.3390/md12105174
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发表时间：
2014-10-14
期刊：
影响因子：
5.4
通讯作者：
Lee TK
中科院分区：
文献类型：
--
作者：
Suh SS;Hwang J;Park M;Seo HH;Kim HS;Lee JH;Moh SH;Lee TK
某些光合作用的海洋生物已经进化出通过合成紫外线吸收化合物来抵消紫外线辐射的机制，如霉菌素样氨基酸(MAAs)。在本研究中，从海洋绿藻Chlamydomonas hedleyi的提取物中分离出MAAs，根据它们的保留时间和最大吸收波长，将它们鉴定为紫菜-334、新诺林和霉孢素-甘氨酸(Mycoporine-Gly)。此外，它们的结构经三重四极杆MS/MS确证，通过分析与抗氧化活性、炎症和皮肤老化相关的基因表达水平，研究它们作为紫外线吸收化合物在人成纤维细胞系HaCaT中对紫外线照射的响应。在2，2-二苯基-1-苦皮酰肼(DPPH)测定中，霉孢素-甘氨酸提取物具有较强的抗氧化活性，而其他MAAS则没有。此外，霉孢素-甘氨酸治疗导致COX-2mRNA水平显著降低，这通常是由于皮肤的炎症反应，以浓度依赖的方式增加。此外，在MAAS的存在下，与皮肤老化有关的紫外线抑制基因--前胶原C蛋白酶增强子(PCOLCE)和弹性蛋白--的表达水平与UV-MOCK处理的细胞相同。有趣的是，紫外线照射后总蛋白表达的增加可以被MAAS霉菌球蛋白-甘氨酸和新诺林抑制，但不能抑制紫菜-334。这是第一份研究微藻来源的MAAs在人类细胞中的生物活性的报告。
Certain photosynthetic marine organisms have evolved mechanisms to counteract UV-radiation by synthesizing UV-absorbing compounds, such as mycosporine-like amino acids (MAAs). In this study, MAAs were separated from the extracts of marine green alga Chlamydomonas hedleyi using HPLC and were identified as porphyra-334, shinorine, and mycosporine-glycine (mycosporine-Gly), based on their retention times and maximum absorption wavelengths. Furthermore, their structures were confirmed by triple quadrupole MS/MS. Their roles as UV-absorbing compounds were investigated in the human fibroblast cell line HaCaT by analyzing the expression levels of genes associated with antioxidant activity, inflammation, and skin aging in response to UV irradiation. The mycosporine-Gly extract, but not the other MAAs, had strong antioxidant activity in the 2,2-diphenyl-1-picryhydrazyl (DPPH) assay. Furthermore, treatment with mycosporine-Gly resulted in a significant decrease in COX-2 mRNA levels, which are typically increased in response to inflammation in the skin, in a concentration-dependent manner. Additionally, in the presence of MAAs, the UV-suppressed genes, procollagen C proteinase enhancer (PCOLCE) and elastin, which are related to skin aging, had increased expression levels equal to those in UV-mock treated cells. Interestingly, the increased expression of involucrin after UV exposure was suppressed by treatment with the MAAs mycosporine-Gly and shinorine, but not porphyra-334. This is the first report investigating the biological activities of microalgae-derived MAAs in human cells.
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影响因子：
3
作者：
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