Dynamics of Individual Red Blood Cells Under Shear Flow: A Way to Discriminate Deformability Alterations.
Dynamics of Individual Red Blood Cells Under Shear Flow: A Way to Discriminate Deformability Alterations.
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DOI：
10.3389/fphys.2021.775584
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发表时间：
2021
影响因子：
4
通讯作者：
Viallat A
中科院分区：
文献类型：
--
作者：
Atwell S;Badens C;Charrier A;Helfer E;Viallat A
在这项工作中，我们比较了来自健康和病理供体（镰状细胞病（SCD）或镰状细胞-β-地中海贫血）的低密度和高密度的单个红细胞（RBC）在较高粘度的悬浮介质中的线性剪切流中的运动动力学。在这些条件下，在较低的剪切速率下，双凹面盘状RBC呈现不稳定的翻转运动，其中细胞对称轴线在剪切平面内旋转，在细胞处于其边缘时观察到的轨道角± θ之间来回摇摆。我们发现，对于健康的红细胞，红细胞密度的演变完全取决于红细胞密度，密度较低的红细胞比较轻的红细胞显示较低的红细胞密度值。典型地，在0.08 Pa的剪切应力下，对于平均密度分别为1.097和1.115的RBC，θ具有82和72°的值。令人惊讶的是，我们表明SCD RBC显示出与相同密度的健康RBC相同的翻转进化，表明翻转行为不受SCD病理的影响。当剪切应力进一步增加（高于0.1 Pa）时，健康的红细胞开始过渡到类似流体的运动，称为坦克踩踏，其中红细胞相对于流动具有准恒定的取向，并且膜围绕细胞的质心旋转。这种转变发生在更高的剪切应力（0.2帕以上）的致密细胞。这种向更高应力的转变在SCD RBC的情况下甚至更显著，表明向坦克踩踏状态的转变高度依赖于SCD病理学。实际上，在0.2Pa的剪切应力下，对于密度为1.097的RBC，100%的健康RBC已经转变为坦克踩踏状态，而SCD RBC少于50%。我们将观察到的动力学差异与文献中报道的密度和SCD病理学方面的RBC机械特性的改变相关联。我们的研究结果表明，它可能是可能的，以开发简单的非侵入性检测的基础上的红细胞在剪切流中的运动，并依赖于这种毫流体方法的诊断目的。
In this work, we compared the dynamics of motion in a linear shear flow of individual red blood cells (RBCs) from healthy and pathological donors (Sickle Cell Disease (SCD) or Sickle Cell-β-thalassemia) and of low and high densities, in a suspending medium of higher viscosity. In these conditions, at lower shear rates, biconcave discocyte-shaped RBCs present an unsteady flip-flopping motion, where the cell axis of symmetry rotates in the shear plane, rocking to and fro between an orbital angle ±ϕ observed when the cell is on its edge. We show that the evolution of ϕ depends solely on RBC density for healthy RBCs, with denser RBCs displaying lower ϕ values than the lighter ones. Typically, at a shear stress of 0.08 Pa, ϕ has values of 82 and 72° for RBCs with average densities of 1.097 and 1.115, respectively. Surprisingly, we show that SCD RBCs display the same ϕ-evolution as healthy RBCs of same density, showing that the flip-flopping behavior is unaffected by the SCD pathology. When the shear stress is increased further (above 0.1 Pa), healthy RBCs start going through a transition to a fluid-like motion, called tank-treading, where the RBC has a quasi-constant orientation relatively to the flow and the membrane rotates around the center of mass of the cell. This transition occurs at higher shear stresses (above 0.2 Pa) for denser cells. This shift toward higher stresses is even more remarkable in the case of SCD RBCs, showing that the transition to the tank-treading regime is highly dependent on the SCD pathology. Indeed, at a shear stress of 0.2 Pa, for RBCs with a density of 1.097, 100% of healthy RBCs have transited to the tank-treading regime vs. less than 50% SCD RBCs. We correlate the observed differences in dynamics to the alterations of RBC mechanical properties with regard to density and SCD pathology reported in the literature. Our results suggest that it might be possible to develop simple non-invasive assays for diagnosis purpose based on the RBC motion in shear flow and relying on this millifluidic approach.
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影响因子：
13.3
作者：
Gambhire, Priya;Atwell, Scott;Charrier, Anne
通讯作者：
Charrier, Anne
影响因子：
4.6
作者：
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56.9
作者：
FISCHER, TM;STOHRLIESEN, M;SCHMIDSCHONBEIN, H
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SCHMIDSCHONBEIN, H
影响因子：
15.9
作者：
CHARACHE, S;CONLEY, CL;SPURRELL, JR
通讯作者：
SPURRELL, JR
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3.4
作者：
Fedosov, Dmitry A.;Caswell, Bruce;Karniadakis, George Em
通讯作者：
Karniadakis, George Em