Rhinacanthus nasutus protects cultured neuronal cells against hypoxia induced cell death.
Rhinacanthus nasutus protects cultured neuronal cells against hypoxia induced cell death.
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DOI：
10.3390/molecules16086322
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发表时间：
2011-07-26
期刊：
影响因子：
--
通讯作者：
Tencomnao T
中科院分区：
文献类型：
--
作者：
Brimson JM;Tencomnao T
鼻刺花Rhinacanthus nasutus（L.）Kurz（菊科）是一种原产于泰国和东南亚的草药，以其抗氧化特性而闻名。缺氧导致细胞中活性氧的增加，是神经元损伤的主要原因。由缺氧引起的细胞死亡与许多神经退行性疾病有关，包括某些形式的痴呆和中风，以及活性氧物质的积累，活性氧物质的积累可导致疾病，如亨廷顿病、帕金森病和阿尔茨海默病。在这项研究中，我们使用了一个密闭的培养容器和三菱气体公司的anaeropack沿着与MTT测定，LDH测定和台盼蓝排斥测定，以显示1和10 μg mL−1的R. nasutus能够显著防止HT-22细胞在缺氧条件下的死亡，并且在正常条件下0.1至10 µg mL−1对HT-22没有毒性作用，而100 µg mL−1降低HT-22细胞增殖。我们还用H2 DCFDA染色显示R.鼻渊能减少HT-22细胞活性氧的产生。
Rhinacanthus nasutus (L.) Kurz (Acanthaceae) is an herb native to Thailand and Southeast Asia, known for its antioxidant properties. Hypoxia leads to an increase in reactive oxygen species in cells and is a leading cause of neuronal damage. Cell death caused by hypoxia has been linked with a number of neurodegenerative diseases including some forms of dementia and stroke, as well as the build up of reactive oxygen species which can lead to diseases such as Huntington’s disease, Parkinson’s disease and Alzeheimer’s disease. In this study we used an airtight culture container and the Mitsubishi Gas Company anaeropack along with the MTT assay, LDH assay and the trypan blue exlusion assay to show that 1 and 10 µg mL−1 root extract of R. nasutus is able to significantly prevent the death of HT-22 cells subjected to hypoxic conditions, and 0.1 to 10 µg mL−1 had no toxic effect on HT-22 under normal conditions, whereas 100 µg mL−1 reduced HT-22 cell proliferation. We also used H2DCFDA staining to show R. nasutus can reduce reactive oxygen species production in HT-22 cells.
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影响因子：
82.9
作者：
Eriksson, PS;Perfilieva, E;Gage, FH
通讯作者：
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DOI：
10.1126/stke.2252004re5
发表时间：
2004-03-16
期刊：
Science's STKE : signal transduction knowledge environment
影响因子：
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