Cytoskeletal reorganization during early mammalian development: analysis using embedment-free sections.

Cytoskeletal reorganization during early mammalian development: analysis using embedment-free sections.
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早期哺乳动物发育过程中的细胞骨架重组:使用无包埋切片进行分析。

DOI:
10.1016/0012-1606(86)90265-4
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发表时间:
1986
影响因子:
2.7
通讯作者:
McGaughey,RW
McGaughey,RW
中科院分区:
生物学3区
文献类型:
--
作者:
Capco,DG;McGaughey,RW

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我们已经研究了细胞骨架重组早期胚胎发育过程中的仓鼠采用洗涤剂提取,以消除可溶性成分的胚胎,揭示了潜在的结构网络。这个程序允许检查皮质细胞骨架和卵内部的细胞骨架。卵和胚胎的切片用可去除的包埋介质二甘醇二硬脂酸酯制备用于透射电子显微镜,其允许比常规包埋程序更厚的切片,从而为研究组织提供更多的空间线索。受精后,细胞骨架在致密化时和胚泡期再次重组。这些细胞骨架重组被认为是卵裂球极性假说和细胞骨架与早期胚胎发育的关系。
We have examined cytoskeletal reorganization during early embryonic development in the hamster by employing detergent extraction to remove soluble components of the embryos and reveal the underlying structural network. This procedure allows examination of both the cortical cytoskeleton and the cytoskeleton of the egg interior. Sections of eggs and embryos were prepared for transmission electron microscopy with the removable embedding medium, diethylene glycol disterate which allows thicker sections than conventional embedment procedures thereby providing more spatial cues for studying organization. The cytoskeleton reorganizes after fertilization, at the time of compaction and again at the blastocyst stage. These cytoskeletal reorganizations are considered in terms of the blastomere polarity hypothesis and the involvement of the cytoskeleton with early embryonic development.
细胞角蛋白多肽在小鼠卵母细胞和植入前胚胎中的表达。
DOI: --
发表时间: 1983
影响因子: 2.7
作者:
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通讯作者: I. Virtanen
神经胶质形状和细胞骨架蛋白合成。
DOI: 10.1007/bf00966796
发表时间: 1992
影响因子: 4.4
作者:
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通讯作者: Edwards,NA
DOI: 10.1016/0092-8674(82)90446-9
发表时间: 1982-01-01
期刊: CELL
影响因子: 64.5
作者:
CAPCO, DG;WAN, KM;PENMAN, S
通讯作者: PENMAN, S
大鼠和小鼠胚胎压缩时细胞质极性形成。
DOI: --
发表时间: 1981
期刊: Journal of embryology and experimental morphology
影响因子: --
作者:
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通讯作者: By W. J. D. Reeve
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DOI: --
发表时间: 1980
期刊:
影响因子: --
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通讯作者: B. Nilsson