Characterization of the abomasal transcriptome for mechanisms of resistance to gastrointestinal nematodes in cattle.
Characterization of the abomasal transcriptome for mechanisms of resistance to gastrointestinal nematodes in cattle.
复制标题
鲍马斯转录组的表征是牛对胃肠道线虫的抗性机制。
DOI：
10.1186/1297-9716-42-114
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发表时间：
2011-11-30
影响因子：
4.4
通讯作者：
Capuco AV
中科院分区：
文献类型：
--
作者：
Li RW;Rinaldi M;Capuco AV
使用 RNA-seq 在每个样本 2370 万个序列的深度上评估了寄生虫敏感和抗寄生虫安格斯牛的真胃转录组对胃肠道寄生虫的反应。通过粪便虫卵计数评估，这些牛表现出明显不同的抗性表型。 23 632 个牛基因中大约 65.3% 在胃底真胃中表达。其中，13 758 个基因在所有测试样本中均表达，可能代表牛皱胃转录组的核心成分。转录本最丰富的基因（BT14427），占转录组序列的10.4%，位于29号染色体上，功能未知。此外，PIGR (1.6%)、补体 C3 (0.7%) 和免疫球蛋白 J 链 (0.5%) 是转录组中最丰富的转录本。在 203 个受影响的基因中，有 64 个基因在严格的临界值 (FDR < 5%) 下在抗性动物中显着过度表达。在鉴定的 94 224 个剪接点中，有 133 个是独特存在的：90 个仅在抗性动物中观察到，43 个仅在易感动物中存在。所研究基因的基因本体论 (GO) 富集揭示了与脂质代谢的关联，这一点已通过独立途径分析得到证实。 FXR/RXR 激活、LXR/RXR 激活、LPS/IL-1 介导的 RXR 功能抑制和花生四烯酸代谢等多种途径在抗性动物中受到影响，这些途径可能参与牛寄生虫抗性的发展。我们的研究结果为了解宿主对胃肠道线虫感染的免疫力的发展提供了见解，并将有助于理解宿主抵抗的潜在机制。
The response of the abomasal transcriptome to gastrointestinal parasites was evaluated in parasite-susceptible and parasite-resistant Angus cattle using RNA-seq at a depth of 23.7 million sequences per sample. These cattle displayed distinctly separate resistance phenotypes as assessed by fecal egg counts. Approximately 65.3% of the 23 632 bovine genes were expressed in the fundic abomasum. Of these, 13 758 genes were expressed in all samples tested and likely represent core components of the bovine abomasal transcriptome. The gene (BT14427) with the most abundant transcript, accounting for 10.4% of sequences in the transcriptome, is located on chromosome 29 and has unknown functions. Additionally, PIGR (1.6%), Complement C3 (0.7%), and Immunoglobulin J chain (0.5%) were among the most abundant transcripts in the transcriptome. Among the 203 genes impacted, 64 were significantly over-expressed in resistant animals at a stringent cutoff (FDR < 5%). Among the 94 224 splice junctions identified, 133 were uniquely present: 90 were observed only in resistant animals, and 43 were present only in susceptible animals. Gene Ontology (GO) enrichment of the genes under study uncovered an association with lipid metabolism, which was confirmed by an independent pathway analysis. Several pathways, such as FXR/RXR activation, LXR/RXR activation, LPS/IL-1 mediated inhibition of RXR function, and arachidonic acid metabolism, were impacted in resistant animals, which are potentially involved in the development of parasite resistance in cattle. Our results provide insights into the development of host immunity to gastrointestinal nematode infection and will facilitate understanding of mechanism underlying host resistance.
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影响因子：
4.4
作者：
French AT;Knight PA;Smith WD;Pate JA;Miller HR;Pemberton AD
通讯作者：
Pemberton AD
影响因子：
4.4
作者：
Borges, Celso Luiz;Cecchini, Rubens;Pinge-Filho, Phileno
通讯作者：
Pinge-Filho, Phileno
影响因子：
2.6
作者：
BARGER, IA;GIBBS, HC
通讯作者：
GIBBS, HC
影响因子：
56.9
作者：
de Urquiza, AM;Liu, SY;Perlmann, T
通讯作者：
Perlmann, T
影响因子：
2.6
作者：
COOP, RL;SYKES, AR;ANGUS, KW
通讯作者：
ANGUS, KW