Expression of three intelectins in sheep and response to a Th2 environment.
Expression of three intelectins in sheep and response to a Th2 environment.
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DOI：
10.1051/vetres/2009035
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发表时间：
2009-11
影响因子：
4.4
通讯作者：
Pemberton AD
中科院分区：
文献类型：
--
作者：
French AT;Knight PA;Smith WD;Pate JA;Miller HR;Pemberton AD
克隆了绵羊intelectin 1和绵羊intelectin 3（sITLN 1和sITLN 3）基因，并进行了序列测定。sITLN 1和sITLN 3的氨基酸序列与已克隆的绵羊intelectin 2（sITLN 2）的同源性分别为86%和91%。sITLN 1和sITLN 3在绵羊皱胃、肺、结肠和胃淋巴结、末端直肠、皮肤、空肠、肠系膜淋巴结、回肠派伊尔集合淋巴结、脑、肾、肝、脾、皮肤、耳廓、心脏和卵巢等组织中均有表达。在正常绵羊组织中，sITLN 2转录本的表达仅限于真胃粘膜。使用非选择性鸡抗-intelectin抗体，组织intelectin蛋白在皱胃中的粘液颈细胞、结肠中的粘液细胞、回肠中的游离粘液、肺中的杯状细胞、小肠上皮和刷状缘、皮肤的表皮层和皮肤皮脂腺中被证明。在已知诱导Th 2应答的绵羊中的两种线虫感染中检查三种sITLN转录物的表达; Teladorsagia circumcincta挑战感染模型和Dictyocaulus filaria自然感染。这三种sITLN在未感染的未感染羔羊中均不存在，而在T.包皮环切瘤挑战感染。sITLN 2和sITLN 3在D.丝虫自然感染。在反刍动物对线虫感染的粘膜反应中，肠凝集素可能发挥重要作用。
Sheep intelectin1 and sheep intelectin3 (sITLN1 and sITLN3) were cloned and sequenced. The amino acid sequences of sITLN1 and sITLN3 shared 86% and 91% homology with the previously cloned sheep intelectin2 (sITLN2), respectively. Expression of sITLN1 and sITLN3 transcript was demonstrated in abomasum, lung, colon and gastric lymph node, terminal rectum, skin, jejunum, mesenteric lymph node, ileal peyer’s patches, brain, kidney, liver, spleen, skin, ear pinna, heart and ovary in normal sheep tissues. sITLN2 transcript expression was restricted to the abomasal mucosa in normal sheep tissues. Using a non selective chicken anti-intelectin antibody, tissue intelectin protein was demonstrated in mucus neck cells in the abomasum, mucus cells in the colon, free mucus in ileum, goblet cells in the lung, small intestinal epithelium and brush border, epidermal layer of the skin and skin sebaceous glands. The expression of the three sITLN transcripts was examined in two nematode infections in sheep known to induce a Th2 response; a Teladorsagia circumcincta challenge infection model and a Dictyocaulus filaria natural infection. The three sITLN were absent in unchallenged naïve lambs and present in the abomasal mucosa of both naïve and immune lambs following T. circumcincta challenge infection. Upregulation of sITLN2 and sITLN3 was shown in sheep lung following D. filaria natural infection. Intelectins may play an important role in the mucosal response to nematode infections in ruminants.
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DOI：
10.1016/s1095-6433(03)00269-1
发表时间：
2004-01-01
影响因子：
2.3
作者：
Chang, BY;Peavy, TR;Hedrick, JL
通讯作者：
Hedrick, JL
DOI：
10.1016/j.bbaexp.2005.11.005
发表时间：
2005-12-30
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Schäffler, A;Neumeier, A;Büchler, C
通讯作者：
Büchler, C
影响因子：
3.5
作者：
Suzuki, YA;Lönnerdal, B
通讯作者：
Lönnerdal, B
影响因子：
2.4
作者：
Blease, Stacey C.;French, Anne T.;Pemberton, Alan D.
通讯作者：
Pemberton, Alan D.
影响因子：
5.3
作者：
Wali, A;Morin, PJ;Pass, HI
通讯作者：
Pass, HI