Transient and Prolonged Response of Chicken Cecum Mucosa to Colonization with Different Gut Microbiota.
Transient and Prolonged Response of Chicken Cecum Mucosa to Colonization with Different Gut Microbiota.
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DOI：
10.1371/journal.pone.0163932
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Rychlik I
中科院分区：
文献类型：
--
作者：
Volf J;Polansky O;Varmuzova K;Gerzova L;Sekelova Z;Faldynova M;Babak V;Medvecky M;Smith AL;Kaspers B;Velge P;Rychlik I
本研究用来自不同日龄母鸡的盲肠内容物接种刚孵化的雏鸡，测定盲肠中蛋白质和基因的表达。超过250个蛋白质表现出对微生物区系接种的改变表达水平。其中ISG12-2、OASL、ES1、LYG2、DMBT1-L、CDD、ANGPTL6、B2M、CUZD1IgM和Ig lambda链的诱导作用最为显著。其中，ISG12-2、ES1和这两种免疫球蛋白在无菌鸡中的表达水平低于常规鸡。相反，CELA2A、BRT-2、ALDH1A1、ADH1C、AKR1B1L、HEXB、ALDH2、ALDOB、CALB1和TTR在接种微生物区系后表达水平较低。当给雏鸡注射来自不同年龄捐赠者的微生物群制剂时，接受者对接种的反应也不同于自然定居鸟类的反应谱。例如，B2M、CUZD1和CELA2A对4周龄或40周龄母鸡接种微生物区系的反应不同。新孵化雏鸡接种后6周即可记录到基因表达的增强或降低。为了鉴定可能直接与微生物区系相互作用的蛋白质，我们鉴定了与微生物区系共纯化的鸡蛋白，并鉴定了一系列宿主蛋白，包括CDD、ANGPTL6、DMBT1-L、MEP1a和Ig lambda。我们认为，诱导ISG12-2可减少宿主细胞对定植共生微生物区系的凋亡，而CDD、ANGPTL6、DMBT1-L、MEP1a和Ig lambda减少腔微生物区系与肠道上皮细胞的接触，从而减轻炎症反应。
In this study we determined protein and gene expression in the caeca of newly hatched chickens inoculated with cecal contents sourced from hens of different ages. Over 250 proteins exhibited modified expression levels in response to microbiota inoculation. The most significant inductions were observed for ISG12-2, OASL, ES1, LYG2, DMBT1-L, CDD, ANGPTL6, B2M, CUZD1, IgM and Ig lambda chain. Of these, ISG12-2, ES1 and both immunoglobulins were expressed at lower levels in germ-free chickens compared to conventional chickens. In contrast, CELA2A, BRT-2, ALDH1A1, ADH1C, AKR1B1L, HEXB, ALDH2, ALDOB, CALB1 and TTR were expressed at lower levels following inoculation of microbiota. When chicks were given microbiota preparations from different age donors, the recipients mounted differential responses to the inoculation which also differed from the response profile in naturally colonised birds. For example, B2M, CUZD1 and CELA2A responded differently to the inoculation with microbiota of 4- or 40-week-old hens. The increased or decreased gene expression could be recorded 6 weeks after the inoculation of newly hatched chickens. To characterise the proteins that may directly interact with the microbiota we characterised chicken proteins that co-purified with the microbiota and identified a range of host proteins including CDD, ANGPTL6, DMBT1-L, MEP1A and Ig lambda. We propose that induction of ISG12-2 results in reduced apoptosis of host cells exposed to the colonizing commensal microbiota and that CDD, ANGPTL6, DMBT1-L, MEP1A and Ig lambda reduce contact of luminal microbiota with the gut epithelium thereby reducing the inflammatory response.
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影响因子：
4.4
作者：
Matulova M;Varmuzova K;Sisak F;Havlickova H;Babak V;Stejskal K;Zdrahal Z;Rychlik I
通讯作者：
Rychlik I
影响因子：
5.4
作者：
Madsen, J;Tornoe, I;Holmskov, U
通讯作者：
Holmskov, U
影响因子：
4.2
作者：
Mariat D;Firmesse O;Levenez F;Guimarăes V;Sokol H;Doré J;Corthier G;Furet JP
通讯作者：
Furet JP
影响因子：
4.1
作者：
Ligtenberg, AJM;Bikker, FJ;Amerongen, AVN
通讯作者：
Amerongen, AVN
影响因子：
8
作者：
Cheriyath, V.;Kuhns, M. A.;Borden, E. C.
通讯作者：
Borden, E. C.