The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age.
The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age.
复制标题
人体微生物区系的菌丝/类杆菌比率随年龄的变化而变化。
DOI：
10.1186/1471-2180-9-123
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发表时间：
2009-06-09
期刊：
影响因子：
4.2
通讯作者：
Furet JP
中科院分区：
文献类型：
--
作者：
Mariat D;Firmesse O;Levenez F;Guimarăes V;Sokol H;Doré J;Corthier G;Furet JP
在人类中，肠道微生物群通过提供能量、营养和免疫保护在维持宿主健康方面起着重要作用。应用目前的分子方法是必要的，以克服经典的培养技术的局限性，以获得准确的描述的微生物群组成。在这里，我们报告了三个年龄组的人类粪便微生物群的比较评估：婴儿，成人和老年人。我们证明了人类肠道微生物群从出生到成年都经历了成熟，并随着年龄的增长而进一步改变。通过定量PCR（qPCR）评估主要细菌群轻盈梭菌、球状梭菌、拟杆菌、双歧杆菌、乳杆菌和大肠杆菌的计数。通过比较物种多样性概况，我们观察到人类粪便微生物群中与年龄相关的变化。婴儿的微生物群通常以低水平的细菌总数为特征。C. leptum和C.球孢子菌在婴儿的微生物群中高度代表，而老年受试者表现出高水平的E。coli和Bacteroidetes。我们观察到厚壁菌门和拟杆菌门的比例在不同的生命阶段发生变化。对于婴儿、成人和老年人，我们测量的比率分别为0.4、10.9和0.6。在这项工作中，我们证实了qPCR是研究多样和复杂粪便微生物群的强大技术。我们的工作表明，粪便微生物群的组成在整个生命过程中不断演变，从幼儿到老年。
In humans, the intestinal microbiota plays an important role in the maintenance of host health by providing energy, nutrients, and immunological protection. Applying current molecular methods is necessary to surmount the limitations of classical culturing techniques in order to obtain an accurate description of the microbiota composition. Here we report on the comparative assessment of human fecal microbiota from three age-groups: infants, adults and the elderly. We demonstrate that the human intestinal microbiota undergoes maturation from birth to adulthood and is further altered with ageing. The counts of major bacterial groups Clostridium leptum, Clostridium coccoides, Bacteroidetes, Bifidobacterium, Lactobacillus and Escherichia coli were assessed by quantitative PCR (qPCR). By comparing species diversity profiles, we observed age-related changes in the human fecal microbiota. The microbiota of infants was generally characterized by low levels of total bacteria. C. leptum and C. coccoides species were highly represented in the microbiota of infants, while elderly subjects exhibited high levels of E. coli and Bacteroidetes. We observed that the ratio of Firmicutes to Bacteroidetes evolves during different life stages. For infants, adults and elderly individuals we measured ratios of 0.4, 10.9 and 0.6, respectively. In this work we have confirmed that qPCR is a powerful technique in studying the diverse and complex fecal microbiota. Our work demonstrates that the fecal microbiota composition evolves throughout life, from early childhood to old age.
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