Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis.
Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis.
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DOI：
10.1186/1297-9716-44-37
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发表时间：
2013-05-20
影响因子：
4.4
通讯作者：
Rychlik I
中科院分区：
文献类型：
--
作者：
Matulova M;Varmuzova K;Sisak F;Havlickova H;Babak V;Stejskal K;Zdrahal Z;Rychlik I
鸡对沙门氏菌感染的免疫反应特征通常局限于细胞因子、趋化因子或抗菌肽编码基因的表达的量化。然而，在感染鸡的盲肠中发生的过程可能要多样化得多。因此，在本研究中，我们对感染肠炎沙门氏菌后鸡盲肠的转录组和蛋白质组进行了研究。结合454焦磷酸测序、蛋白质质谱学和实时定量聚合酶链式反应，我们鉴定了肠炎沙门氏菌感染后48个下调和56个上调的鸡基因。最易诱导的基因是编码MMP7的基因，在感染后第9天表现出5952倍的诱导。免疫球蛋白Ig G、IrG1、SAA、ExFABP、IL-22、TRAP6、MRP126、干扰素γ、诱导型一氧化氮合酶、Es1、IL-1β、LYG2、IFIT5、IL-17、AVD、AH221和SERPIN B的诱导倍数均在100倍以上。最后，上述信号依赖于肠炎沙门氏菌中是否存在SPI1编码的III型分泌系统。炎症持续2周后，炎症基因的表达恢复到基础水平，而免疫球蛋白A和免疫球蛋白的表达增加。这表明免疫球蛋白在感染后盲肠内稳态的恢复中起着重要作用。
The characterization of the immune response of chickens to Salmonella infection is usually limited to the quantification of expression of genes coding for cytokines, chemokines or antimicrobial peptides. However, processes occurring in the cecum of infected chickens are likely to be much more diverse. In this study we have therefore characterized the transcriptome and proteome in the chicken cecum after infection with Salmonella Enteritidis. Using a combination of 454 pyrosequencing, protein mass spectrometry and quantitative real-time PCR, we identified 48 down- and 56 up-regulated chicken genes after Salmonella Enteritidis infection. The most inducible gene was that coding for MMP7, exhibiting a 5952 fold induction 9 days post-infection. An induction of greater than 100 fold was observed for IgG, IRG1, SAA, ExFABP, IL-22, TRAP6, MRP126, IFNγ, iNOS, ES1, IL-1β, LYG2, IFIT5, IL-17, AVD, AH221 and SERPIN B. Since prostaglandin D2 synthase was upregulated and degrading hydroxyprostaglandin dehydrogenase was downregulated after the infection, prostaglandin must accumulate in the cecum of chickens infected with Salmonella Enteritidis. Finally, above mentioned signaling was dependent on the presence of a SPI1-encoded type III secretion system in Salmonella Enteritidis. The inflammation lasted for 2 weeks after which time the expression of the “inflammatory” genes returned back to basal levels and, instead, the expression of IgA and IgG increased. This points to an important role for immunoglobulins in the restoration of homeostasis in the cecum after infection.
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鸡肉肠肠肠肠肠肠肠肠球菌的五个主要致病岛（SPI-1至SPI-5）的毒力潜力用于鸡。
影响因子：
4.2
作者：
Rychlik I;Karasova D;Sebkova A;Volf J;Sisak F;Havlickova H;Kummer V;Imre A;Szmolka A;Nagy B
通讯作者：
Nagy B
影响因子：
2.8
作者：
Beal, RK;Powers, C;Smith, AL
通讯作者：
Smith, AL
DOI：
10.1073/pnas.0307317101
发表时间：
2004-02-17
影响因子：
11.1
作者：
Suzuki, K;Meek, B;Fagarasan, S
通讯作者：
Fagarasan, S
影响因子：
48
作者：
Wisniewski, Jacek R.;Zougman, Alexandre;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
2.5
作者：
Zhao, Shu-Hong;Kuhar, Daniel;Tuggle, Christopher K.
通讯作者：
Tuggle, Christopher K.