Detection of SARS-CoV-2 in wastewater in Japan during a COVID-19 outbreak.
Detection of SARS-CoV-2 in wastewater in Japan during a COVID-19 outbreak.
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DOI：
10.1016/j.scitotenv.2020.143578
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发表时间：
2021-03-01
期刊：
影响因子：
--
通讯作者：
Honda R
中科院分区：
文献类型：
--
作者：
Hata A;Hara-Yamamura H;Meuchi Y;Imai S;Honda R
在几个国家的废水样本中发现了严重急性呼吸系统综合征冠状病毒2型（SARS-CoV-2）。基于水的流行病学（WBE）对于COVID-19爆发的早期预警可能是有效的。在这项研究中，调查了废水样本中SARS-CoV-2 RNA的存在情况，并将其与日本COVID-19爆发期间研究地区确诊的COVID-19病例数量进行了比较。从日本石川县和富山县的五个污水处理厂总共收集了45个进水废水样品。在研究期间，这些县的确诊COVID-19病例数从每10万人0. 3和0增加到每10万人20例以上。使用几种基于PCR的检测方法检测样本中的SARS-CoV-2核糖核酸（RNA）。根据三种定量RT-PCR检测中的至少一种，在45份样本中，21份样本对SARS-CoV-2呈阳性。当每个都道府县每10万人中确诊的SARS-CoV-2病例总数超过10例时，检测频率会增加;然而，即使该数字低于1.0，SARS-CoV-2也可以以低频率检测到。废水中的SARS-CoV-2可以在流行病的早期阶段被检测到，即使确诊病例的数量可能低估了实际病例的数量。这表明WBE方法可能成为日本COVID-19爆发的早期预警。
The presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater samples has been documented in several countries. Wastewater-based epidemiology (WBE) is potentially effective for early warning of a COVID-19 outbreak. In this study, presence of SARS-CoV-2 RNA in wastewater samples was investigated and was compared with the number of the confirmed COVID-19 cases in the study area during COVID-19 outbreak in Japan. In total, 45 influent wastewater samples were collected from five wastewater treatment plants in Ishikawa and Toyama prefectures in Japan. During the study period, the numbers of confirmed COVID-19 cases in these prefectures increased from 0.3 and 0 to >20 per 100,000 people. SARS-CoV-2 ribonucleic acid (RNA) in the samples was detected using several PCR-based assays. Of the 45 samples, 21 were positive for SARS-CoV-2 according to at least one of the three quantitative RT-PCR assays. The detection frequency increased when the number of total confirmed SARS-CoV-2 cases in 100,000 people exceeded 10 in each prefecture; however, SARS-CoV-2 could also be detected at a low frequency even when the number was below 1.0. SARS-CoV-2 in wastewater could be detected in the early stage of the epidemic, even if the number of confirmed cases potentially underestimates the actual numbers of cases. This suggests that WBE approach can potentially act as an early warning of COVID-19 outbreaks in Japan.
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